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Journal of Bacteriology, April 2003, p. 2374-2378, Vol. 185, No. 7
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.7.2374-2378.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Biofilm Formation by Hyperpiliated Mutants of Pseudomonas aeruginosa

Poney Chiang and Lori L. Burrows*

Centre for Infection and Biomaterials Research, Hospital for Sick Children, and Department of Surgery, University of Toronto, Toronto, Canada

Received 4 October 2002/ Accepted 19 December 2002

Under static growth conditions, hyperpiliated, nontwitching pilT and pilU mutants of Pseudomonas aeruginosa formed dense biofilms, showing that adhesion, not twitching motility, is necessary for biofilm initiation. Under flow conditions, the pilT mutant formed mushroom-like structures larger than those of the wild type but the pilU mutant was defective in biofilm formation. Therefore, twitching motility affects the development of biofilm structure, possibly through modulation of detachment.

* Corresponding author. Mailing address: Centre for Infection and Biomaterials Research, 7142A Elm Wing, Hospital for Sick Children Research Institute, 555 University Ave., Toronto ON M5G 1X8, Canada. Phone: (416) 813-6293. Fax: (416) 813-6461. E-mail: lori.burrows{at}sickkids.ca.

Journal of Bacteriology, April 2003, p. 2374-2378, Vol. 185, No. 7
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.7.2374-2378.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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