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Hydrogen-Oxidizing Capabilities of Helicobacter hepaticus and In Vivo Availability of the Substrate

Robert J. Maier,^* Jonathan Olson, and Adriana Olczak

Department of Microbiology, The University of Georgia, Athens, Georgia 30602

Received 10 October 2002/ Accepted 27 January 2003

Hydrogen-oxidizing hydrogenase activity was detected in Helicobacter hepaticus and compared to the activity in Helicobacter pylori for characteristics associated with hydrogen uptake respiratory hydrogenases. Intact whole cells could couple H₂ oxidation to oxygen uptake, and no H₂ uptake was observed without oxygen available to complete the respiratory pathway. The H. hepaticus enzyme coupled H₂ oxidation to reduction of many positive potential acceptors, and it underwent anaerobic or reductive activation. H. hepaticus had a strong affinity for molecular H₂ (apparent K_m of 2.5 µM), and microelectrode measurements on the livers of live mice demonstrated that H₂ is available in the host tissue at levels 20-fold greater than the apparent whole-cell K_m value.

Present address: Dept. of Microbiology, North Carolina State University, Raleigh, NC 27695.

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