This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sampson, S. L. Articles by van Helden, P. D. Search for Related Content PubMed PubMed Citation Articles by Sampson, S. L. Articles by van Helden, P. D.

IS6110-Mediated Deletion Polymorphism in the Direct Repeat Region of Clinical Isolates of Mycobacterium tuberculosis

MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, Faculty of Health Sciences, Stellenbosch University, Tygerberg 7505, South Africa

Received 23 September 2002/ Accepted 17 January 2003

This study investigates the phenomenon of IS6110-mediated deletion polymorphism in the direct repeat (DR) region of the genome of Mycobacterium tuberculosis. Clinical isolates and their putative predecessors were compared using a combination of DR region restriction fragment length polymorphism, IS6110 DNA fingerprinting, spoligotyping, and DNA sequencing, which allowed the mapping of chromosome structure and deletion junctions. The data suggest that adjacently situated IS6110 elements mediate genome deletion. However, in contrast to previous reports, deletions appear to be mediated by inversely oriented IS6110 elements. This suggests that these events may occur via mechanisms other than RecA-mediated homologous recombination. The results underscore the important role of IS6110-associated deletion hypervariability in driving M. tuberculosis genome evolution.

This article has been cited by other articles:

• Mathema, B., Kurepina, N. E., Bifani, P. J., Kreiswirth, B. N. (2006). Molecular Epidemiology of Tuberculosis: Current Insights. Clin. Microbiol. Rev. 19: 658-685 [Abstract] [Full Text]  
• Kurepina, N., Likhoshvay, E., Shashkina, E., Mathema, B., Kremer, K., van Soolingen, D., Bifani, P., Kreiswirth, B. N. (2005). Targeted Hybridization of IS6110 Fingerprints Identifies the W-Beijing Mycobacterium tuberculosis Strains among Clinical Isolates. J. Clin. Microbiol. 43: 2148-2154 [Abstract] [Full Text]  
• Fournier, P.-E., Zhu, Y., Ogata, H., Raoult, D. (2004). Use of Highly Variable Intergenic Spacer Sequences for Multispacer Typing of Rickettsia conorii Strains. J. Clin. Microbiol. 42: 5757-5766 [Abstract] [Full Text]  
• Aranaz, A., Romero, B., Montero, N., Alvarez, J., Bezos, J., de Juan, L., Mateos, A., Dominguez, L. (2004). Spoligotyping Profile Change Caused by Deletion of a Direct Variable Repeat in a Mycobacterium tuberculosis Isogenic Laboratory Strain. J. Clin. Microbiol. 42: 5388-5391 [Abstract] [Full Text]  
• Viana-Niero, C., de Haas, P. E., van Soolingen, D., Leao, S. C. (2004). Analysis of genetic polymorphisms affecting the four phospholipase C (plc) genes in Mycobacterium tuberculosis complex clinical isolates. Microbiology 150: 967-978 [Abstract] [Full Text]  
• Sampson, S. L., Richardson, M., van Helden, P. D., Warren, R. M. (2004). IS6110-Mediated Deletion Polymorphism in Isogenic Strains of Mycobacterium tuberculosis. J. Clin. Microbiol. 42: 895-898 [Abstract] [Full Text]  
• Mostowy, S., Cousins, D., Behr, M. A. (2004). Genomic Interrogation of the Dassie Bacillus Reveals It as a Unique RD1 Mutant within the Mycobacterium tuberculosis Complex. J. Bacteriol. 186: 104-109 [Abstract] [Full Text]