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Journal of Bacteriology, May 2003, p. 2961-2966, Vol. 185, No. 9
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.9.2961-2966.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Essentiality of clpX, but Not clpP, clpL, clpC, or clpE, in Streptococcus pneumoniae R6

Gregory T. Robertson,1,{dagger} Wai-Leung Ng,1,2 Raymond Gilmour,1 and Malcolm E. Winkler1,2*

Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285,1 Department of Biology, Indiana University, Bloomington, Indiana 474052

Received 16 September 2002/ Accepted 19 February 2003

We show by using a regulated promoter that clpX of Streptococcus pneumoniae R6 is essential, whereas clpP, clpL, clpC, and clpE can be disrupted. The essentiality of clpX was initially missed because of duplication and rearrangement in the region of the chromosome containing clpX. Depletion of ClpX resulted in a rapid loss of viability without overt changes in cell morphology. Essentiality of clpX, but not clpP, has not been reported previously.

* Corresponding author. Mailing address: Department of Biology, Indiana University, Jordan Hall 142, Bloomington, IN 47405. Phone: (317) 508-7812. Fax: (812) 855-6705. E-mail: mwinkler{at}bio.indiana.edu.

{dagger} Present address: Cumbre, Inc., Dallas, TX 75235.

Journal of Bacteriology, May 2003, p. 2961-2966, Vol. 185, No. 9
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.9.2961-2966.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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