This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Perret, S. Articles by Belaich, A. Search for Related Content PubMed PubMed Citation Articles by Perret, S. Articles by Belaich, A.

Production of Heterologous and Chimeric Scaffoldins by Clostridium acetobutylicum ATCC 824

S. Perret,¹ L. Casalot,^1, H.-P. Fierobe,¹ C. Tardif,^1,2 F. Sabathe,³ J.-P. Belaich,^1,2 and A. Belaich^1*

Laboratoire de Bioénergétique et Ingénierie des Protéines, IBSM, Centre National de la Recherche Scientifique,¹ Université de Provence, Marseille,² Centre de Bioingénierie Gilbert Durand, Institut National des Sciences Appliquées, Toulouse, France³

Received 23 May 2003/ Accepted 1 October 2003

Clostridium acetobutylicum ATCC 824 converts sugars and various polysaccharides into acids and solvents. This bacterium, however, is unable to utilize cellulosic substrates, since it is able to secrete very small amounts of cellulosomes. To promote the utilization of crystalline cellulose, the strategy we chose aims at producing heterologous minicellulosomes, containing two different cellulases bound to a miniscaffoldin, in C. acetobutylicum. A first step toward this goal describes the production of miniCipC1, a truncated form of CipC from Clostridium cellulolyticum, and the hybrid scaffoldin Scaf 3, which bears an additional cohesin domain derived from CipA from Clostridium thermocellum. Both proteins were correctly matured and secreted in the medium, and their various domains were found to be functional.

Present address: Laboratoire de Biodépollution et Valorisation des Effluents Gazeux, Institut de Recherche pour le Développement, Marseille, France.

This article has been cited by other articles:

• DURRE, P. (2008). Fermentative Butanol Production: Bulk Chemical and Biofuel. Ann. N. Y. Acad. Sci. 1125: 353-362 [Abstract] [Full Text]  
• Abdou, L., Boileau, C., de Philip, P., Pages, S., Fierobe, H.-P., Tardif, C. (2008). Transcriptional Regulation of the Clostridium cellulolyticum cip-cel Operon: a Complex Mechanism Involving a Catabolite-Responsive Element. J. Bacteriol. 190: 1499-1506 [Abstract] [Full Text]  
• DOI, R. H. (2008). Cellulases of Mesophilic Microorganisms: Cellulosome and Noncellulosome Producers. Ann. N. Y. Acad. Sci. 1125: 267-279 [Abstract] [Full Text]  
• Mingardon, F., Chanal, A., Tardif, C., Bayer, E. A., Fierobe, H.-P. (2007). Exploration of New Geometries in Cellulosome-Like Chimeras. Appl. Environ. Microbiol. 73: 7138-7149 [Abstract] [Full Text]  
• Mingardon, F., Perret, S., Belaich, A., Tardif, C., Belaich, J.-P., Fierobe, H.-P. (2005). Heterologous Production, Assembly, and Secretion of a Minicellulosome by Clostridium acetobutylicum ATCC 824. Appl. Environ. Microbiol. 71: 1215-1222 [Abstract] [Full Text]  
• Demain, A. L., Newcomb, M., Wu, J. H. D. (2005). Cellulase, Clostridia, and Ethanol. Microbiol. Mol. Biol. Rev. 69: 124-154 [Abstract] [Full Text]  
• Perret, S., Belaich, A., Fierobe, H.-P., Belaich, J.-P., Tardif, C. (2004). Towards Designer Cellulosomes in Clostridia: Mannanase Enrichment of the Cellulosomes Produced by Clostridium cellulolyticum. J. Bacteriol. 186: 6544-6552 [Abstract] [Full Text]  
• Cho, H.-Y., Yukawa, H., Inui, M., Doi, R. H., Wong, S.-L. (2004). Production of Minicellulosomes from Clostridium cellulovorans in Bacillus subtilis WB800. Appl. Environ. Microbiol. 70: 5704-5707 [Abstract] [Full Text]