This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brokx, S. J. Articles by Weiner, J. H. Search for Related Content PubMed PubMed Citation Articles by Brokx, S. J. Articles by Weiner, J. H.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 2004, p. 3254-3258, Vol. 186, No. 10
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.10.3254-3258.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genome-Wide Analysis of Lipoprotein Expression in Escherichia coli MG1655

Project CyberCell, Department of Biochemistry,¹ CIHR Membrane Protein Research Group,² Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2H7, Canada³

Received 24 July 2003/ Accepted 3 February 2004

To gain insight into the cell envelope of Escherichia coli grown under aerobic and anaerobic conditions, lipoproteins were examined by using functional genomics. The mRNA expression levels of each of these genes under three growth conditions—aerobic, anaerobic, and anaerobic with nitrate—were examined by using both Affymetrix GeneChip E. coli antisense genome arrays and real-time PCR (RT-PCR). Many genes showed significant changes in expression level. The RT-PCR results were in very good agreement with the microarray data. The results of this study represent the first insights into the possible roles of unknown lipoprotein genes and broaden our understanding of the composition of the cell envelope under different environmental conditions. Additionally, these data serve as a test set for the refinement of high-throughput bioinformatic and global gene expression methods.

This article has been cited by other articles:

• Watanabe, S., Oguchi, Y., Yokota, N., Tokuda, H. (2007). Large-scale preparation of the homogeneous LolA lipoprotein complex and efficient in vitro transfer of lipoproteins to the outer membrane in a LolB-dependent manner. Protein Sci. 16: 2741-2749 [Abstract] [Full Text]  
• Watanabe, S., Matsuyama, S.-i., Tokuda, H. (2006). Roles of the Hydrophobic Cavity and Lid of LolA in the Lipoprotein Transfer Reaction in Escherichia coli. J. Biol. Chem. 281: 3335-3342 [Abstract] [Full Text]  
• Taniguchi, N., Matsuyama, S.-i., Tokuda, H. (2005). Mechanisms Underlying Energy-independent Transfer of Lipoproteins from LolA to LolB, Which Have Similar Unclosed {beta}-Barrel Structures. J. Biol. Chem. 280: 34481-34488 [Abstract] [Full Text]  
• Egler, M., Grosse, C., Grass, G., Nies, D. H. (2005). Role of the Extracytoplasmic Function Protein Family Sigma Factor RpoE in Metal Resistance of Escherichia coli. J. Bacteriol. 187: 2297-2307 [Abstract] [Full Text]  
• Miyadai, H., Tanaka-Masuda, K., Matsuyama, S.-i., Tokuda, H. (2004). Effects of Lipoprotein Overproduction on the Induction of DegP (HtrA) Involved in Quality Control in the Escherichia coli Periplasm. J. Biol. Chem. 279: 39807-39813 [Abstract] [Full Text]