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Journal of Bacteriology, May 2004, p. 3262-3265, Vol. 186, No. 10
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.10.3262-3265.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Nucleotide Transporter of Caedibacter caryophilus Exhibits an Extended Substrate Spectrum Compared to the Analogous ATP/ADP Translocase of Rickettsia prowazekii

Robin M. Daugherty,1 Nicole Linka,2 Jonathon P. Audia,1 Claude Urbany,2 H. Ekkehard Neuhaus,2 and Herbert H. Winkler1*

Laboratory of Molecular Biology, Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile, Alabama 36688,1 Universität Kaiserslautern, Pflanzenphysiologie, Postfach 3049, D-67653, Kaiserslautern, Germany2

Received 26 December 2003/ Accepted 3 February 2004

The two obligate intracellular alphaproteobacteria Rickettsia prowazekii and Caedibacter caryophilus, a human pathogen and a paramecium endosymbiont, respectively, possess transport systems to facilitate ATP uptake from the host cell cytosol. These transport proteins, which have 65% identity at the amino acid level, were heterologously expressed in Escherichia coli, and their properties were compared. The results presented here demonstrate that the caedibacter transporter had a broader substrate than the more selective rickettsial transporter. ATP analogs with modified sugar moieties, dATP and ddATP, inhibited the transport of ATP by the caedibacter transporter but not by the rickettsial transporter. Both transporters were specific for di- and trinucleotides with an adenine base in that adenosine tetraphosphate, AMP, UTP, CTP, and GTP were not competitive inhibitors. Furthermore, the antiporter nature of both transport systems was shown by the dependence of the efflux of [{alpha}-32P]ATP on the influx of substrate (ATP but not dATP for rickettsiae, ATP or dATP for caedibacter).

* Corresponding author. Mailing address: Laboratory of Molecular Biology, Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile, AL 36688. Phone: (251) 460-6108. Fax: (251) 460-7269. E-mail: herbertw{at}sungcg.usouthal.edu.

Journal of Bacteriology, May 2004, p. 3262-3265, Vol. 186, No. 10
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.10.3262-3265.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ast, M., Gruber, A., Schmitz-Esser, S., Neuhaus, H. E., Kroth, P. G., Horn, M., Haferkamp, I. (2009). Diatom plastids depend on nucleotide import from the cytosol. Proc. Natl. Acad. Sci. USA 106: 3621-3626 [Abstract] [Full Text]  
  • Trentmann, O., Jung, B., Neuhaus, H. E., Haferkamp, I. (2008). Nonmitochondrial ATP/ADP Transporters Accept Phosphate as Third Substrate. J. Biol. Chem. 283: 36486-36493 [Abstract] [Full Text]  
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