This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lau, G. H. Y.
Right arrow Articles by Murphy, M. E. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lau, G. H. Y.
Right arrow Articles by Murphy, M. E. P.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 2004, p. 3266-3269, Vol. 186, No. 10
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.10.3266-3269.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Characterization of a Nucleotide-Binding Domain Associated with Neisserial Iron Transport

Gloria H. Y. Lau,1 Ross T. A. MacGillivray,1 and Michael E. P. Murphy1,2*

Department of Biochemistry and Molecular Biology,1 Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada2

Received 22 October 2003/ Accepted 3 February 2004

The fbpABC operon in Neisseria gonorrhoeae encodes an ATP-binding cassette transporter required for iron uptake from the host ferric binding proteins. The gene for the nucleotide-binding domain (fbpC) expressed in Escherichia coli has intrinsic ATPase activity (0.5 mmol/min/mg) uncoupled from the iron transport process. The FbpC E164D mutant is found to have a 10-fold reduction in specific activity. FbpC is covalently modified by 8-azido-[{gamma}32P]ATP, indicating that FbpC is a functional ATPase that likely combines with FbpB to form a ferric iron transporter.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of British Columbia, #300 6174 University Blvd., Vancouver, BC V6T 1Z3, Canada. Phone: (604) 822-8022. Fax: (604) 822-6041. E-mail: memurphy{at}interchange.ubc.ca.

Journal of Bacteriology, May 2004, p. 3266-3269, Vol. 186, No. 10
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.10.3266-3269.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»