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Journal of Bacteriology, May 2004, p. 3270-3273, Vol. 186, No. 10
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.10.3270-3273.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Isolation and Characterization of a Generalized Transducing Phage for Pseudomonas aeruginosa Strains PAO1 and PA14

Dartmouth College and Department of Microbiology and Immunology,¹ Dartmouth Medical School, Hanover, New Hampshire 03755,⁴ Faculty of Biological Science, The University of Tulsa, Tulsa, Oklahoma 74104,² Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115³

Received 29 October 2003/ Accepted 2 February 2004

A temperate, type IV pilus-dependent, double-stranded DNA bacteriophage named DMS3 was isolated from a clinical strain of Pseudomonas aeruginosa. A clear-plaque variant of this bacteriophage was isolated. DMS3 is capable of mediating generalized transduction within and between P. aeruginosa strains PA14 and PAO1, thus providing a useful tool for the genetic analysis of P. aeruginosa.

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