This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Konkel, M. E. Articles by Mickelson, J. Search for Related Content PubMed PubMed Citation Articles by Konkel, M. E. Articles by Mickelson, J.

Secretion of Virulence Proteins from Campylobacter jejuni Is Dependent on a Functional Flagellar Export Apparatus

School of Molecular Biosciences, Washington State University, Pullman, Washington 99164

Received 20 October 2003/ Accepted 19 February 2004

Campylobacter jejuni, a gram-negative motile bacterium, secretes a set of proteins termed the Campylobacter invasion antigens (Cia proteins). The purpose of this study was to determine whether the flagellar apparatus serves as the export apparatus for the Cia proteins. Mutations were generated in five genes encoding three structural components of the flagella, the flagellar basal body (flgB and flgC), hook (flgE2), and filament (flaA and flaB) genes, as well as in genes whose products are essential for flagellar protein export (flhB and fliI). While mutations that affected filament assembly were found to be nonmotile (Mot^–) and did not secrete Cia proteins (S^–), a flaA (flaB⁺) filament mutant was found to be nonmotile but Cia protein secretion competent (Mot^–, S⁺). Complementation of a flaA flaB double mutant with a shuttle plasmid harboring either the flaA or flaB gene restored Cia protein secretion, suggesting that Cia export requires at least one of the two filament proteins. Infection of INT 407 human intestinal cells with the C. jejuni mutants revealed that maximal invasion of the epithelial cells required motile bacteria that are secretion competent. Collectively, these data suggest that the C. jejuni Cia proteins are secreted from the flagellar export apparatus.

This article has been cited by other articles:

• Baqar, S., Applebee, L. A., Gilliland, T. C. Jr., Lee, L. H., Porter, C. K., Guerry, P. (2008). Immunogenicity and Protective Efficacy of Recombinant Campylobacter jejuni Flagellum-Secreted Proteins in Mice. Infect. Immun. 76: 3170-3175 [Abstract] [Full Text]  
• Yun, J., Jeon, B., Barton, Y.-W., Plummer, P., Zhang, Q., Ryu, S. (2008). Role of the DksA-Like Protein in the Pathogenesis and Diverse Metabolic Activity of Campylobacter jejuni. J. Bacteriol. 190: 4512-4520 [Abstract] [Full Text]  
• Malik-Kale, P., Parker, C. T., Konkel, M. E. (2008). Culture of Campylobacter jejuni with Sodium Deoxycholate Induces Virulence Gene Expression. J. Bacteriol. 190: 2286-2297 [Abstract] [Full Text]  
• Castelli, M. E., Fedrigo, G. V., Clementin, A. L., Ielmini, M. V., Feldman, M. F., Vescovi, E. G. (2008). Enterobacterial Common Antigen Integrity Is a Checkpoint for Flagellar Biogenesis in Serratia marcescens. J. Bacteriol. 190: 213-220 [Abstract] [Full Text]  
• Kalischuk, L. D., Inglis, G. D., Buret, A. G. (2007). Strain-dependent induction of epithelial cell oncosis by Campylobacter jejuni is correlated with invasion ability and is independent of cytolethal distending toxin. Microbiology 153: 2952-2963 [Abstract] [Full Text]  
• Kamal, N., Dorrell, N., Jagannathan, A., Turner, S. M., Constantinidou, C., Studholme, D. J., Marsden, G., Hinds, J., Laing, K. G., Wren, B. W., Penn, C. W. (2007). Deletion of a previously uncharacterized flagellar-hook-length control gene fliK modulates the {sigma}54-dependent regulon in Campylobacter jejuni. Microbiology 153: 3099-3111 [Abstract] [Full Text]  
• Salvetti, S., Ghelardi, E., Celandroni, F., Ceragioli, M., Giannessi, F., Senesi, S. (2007). FlhF, a signal recognition particle-like GTPase, is involved in the regulation of flagellar arrangement, motility behaviour and protein secretion in Bacillus cereus. Microbiology 153: 2541-2552 [Abstract] [Full Text]  
• Poly, F., Ewing, C., Goon, S., Hickey, T. E., Rockabrand, D., Majam, G., Lee, L., Phan, J., Savarino, N. J., Guerry, P. (2007). Heterogeneity of a Campylobacter jejuni Protein That Is Secreted through the Flagellar Filament. Infect. Immun. 75: 3859-3867 [Abstract] [Full Text]  
• Parthasarathy, G., Yao, Y., Kim, K. S. (2007). Flagella Promote Escherichia coli K1 Association with and Invasion of Human Brain Microvascular Endothelial Cells. Infect. Immun. 75: 2937-2945 [Abstract] [Full Text]  
• Malik-Kale, P., Raphael, B. H., Parker, C. T., Joens, L. A., Klena, J. D., Quinones, B., Keech, A. M., Konkel, M. E. (2007). Characterization of Genetically Matched Isolates of Campylobacter jejuni Reveals that Mutations in Genes Involved in Flagellar Biosynthesis Alter the Organism's Virulence Potential. Appl. Environ. Microbiol. 73: 3123-3136 [Abstract] [Full Text]  
• McNally, D. J., Aubry, A. J., Hui, J. P. M., Khieu, N. H., Whitfield, D., Ewing, C. P., Guerry, P., Brisson, J.-R., Logan, S. M., Soo, E. C. (2007). Targeted Metabolomics Analysis of Campylobacter coli VC167 Reveals Legionaminic Acid Derivatives as Novel Flagellar Glycans. J. Biol. Chem. 282: 14463-14475 [Abstract] [Full Text]  
• Rawls, J. F., Mahowald, M. A., Goodman, A. L., Trent, C. M., Gordon, J. I. (2007). In vivo imaging and genetic analysis link bacterial motility and symbiosis in the zebrafish gut. Proc. Natl. Acad. Sci. USA 104: 7622-7627 [Abstract] [Full Text]  
• Goon, S., Ewing, C. P., Lorenzo, M., Pattarini, D., Majam, G., Guerry, P. (2006). A {sigma}28-Regulated Nonflagella Gene Contributes to Virulence of Campylobacter jejuni 81-176. Infect. Immun. 74: 769-772 [Abstract] [Full Text]  
• Kazmierczak, M. J., Wiedmann, M., Boor, K. J. (2005). Alternative Sigma Factors and Their Roles in Bacterial Virulence. Microbiol. Mol. Biol. Rev. 69: 527-543 [Abstract] [Full Text]  
• Troisfontaines, P., Cornelis, G. R. (2005). Type III Secretion: More Systems Than You Think. Physiology 20: 326-339 [Abstract] [Full Text]  
• Molofsky, A. B., Shetron-Rama, L. M., Swanson, M. S. (2005). Components of the Legionella pneumophila Flagellar Regulon Contribute to Multiple Virulence Traits, Including Lysosome Avoidance and Macrophage Death. Infect. Immun. 73: 5720-5734 [Abstract] [Full Text]  
• Hickey, T. E., Majam, G., Guerry, P. (2005). Intracellular Survival of Campylobacter jejuni in Human Monocytic Cells and Induction of Apoptotic Death by Cytholethal Distending Toxin. Infect. Immun. 73: 5194-5197 [Abstract] [Full Text]  
• Karlyshev, A. V., Wren, B. W. (2005). Development and Application of an Insertional System for Gene Delivery and Expression in Campylobacter jejuni. Appl. Environ. Microbiol. 71: 4004-4013 [Abstract] [Full Text]  
• Arora, S. K., Neely, A. N., Blair, B., Lory, S., Ramphal, R. (2005). Role of Motility and Flagellin Glycosylation in the Pathogenesis of Pseudomonas aeruginosa Burn Wound Infections. Infect. Immun. 73: 4395-4398 [Abstract] [Full Text]  
• Cummings, L. A., Barrett, S. L. R., Wilkerson, W. D., Fellnerova, I., Cookson, B. T. (2005). FliC-Specific CD4+ T Cell Responses Are Restricted by Bacterial Regulation of Antigen Expression. J. Immunol. 174: 7929-7938 [Abstract] [Full Text]  
• Andersen, M. T., Brondsted, L., Pearson, B. M., Mulholland, F., Parker, M., Pin, C., Wells, J. M., Ingmer, H. (2005). Diverse roles for HspR in Campylobacter jejuni revealed by the proteome, transcriptome and phenotypic characterization of an hspR mutant. Microbiology 151: 905-915 [Abstract] [Full Text]