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Journal of Bacteriology, June 2004, p. 3439-3446, Vol. 186, No. 11
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.11.3439-3446.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The davDT Operon of Pseudomonas putida, Involved in Lysine Catabolism, Is Induced in Response to the Pathway Intermediate {delta}-Aminovaleric Acid

Olga Revelles,1 Manuel Espinosa-Urgel,1 Soeren Molin,2 and Juan L. Ramos1*

Department of Biochemistry and Molecular and Cell Biology of Plants, Estación Experimental del Zaidín, CSIC, E-18008 Granada, Spain,1 Molecular Microbial Ecology Group, BioCentrum DTU, 2800 Lyngby, Denmark2

Received 2 December 2003/ Accepted 17 February 2004

Pseudomonas putida KT2440 is a soil microorganism that attaches to seeds and efficiently colonizes the plant's rhizosphere. Lysine is one of the major compounds in root exudates, and P. putida KT2440 uses this amino acid as a source of carbon, nitrogen, and energy. Lysine is channeled to {delta}-aminovaleric acid and then further degraded to glutaric acid via the action of the davDT gene products. We show that the davDT genes form an operon transcribed from a single {sigma}70-dependent promoter. The relatively high level of basal expression from the davD promoter increased about fourfold in response to the addition of exogenous lysine to the culture medium. However, the true inducer of this operon seems to be {delta}-aminovaleric acid because in a mutant unable to metabolize lysine to {delta}-aminovaleric acid, this compound, but not lysine, acted as an effector. Effective induction of the P. putida PdavD promoter by exogenously added lysine requires efficient uptake of this amino acid, which seems to proceed by at least two uptake systems for basic amino acids that belong to the superfamily of ABC transporters. Mutants in these ABC uptake systems retained basal expression from the davD promoter but exhibited lower induction levels in response to exogenous lysine than the wild-type strain.

* Corresponding author. Mailing address: Estación Experimental del Zaidín, CSIC, Calle Profesor Albareda number 1, E-18008 Granada, Spain. Phone: 34-958-181608. Fax: 34-958-135740. E-mail: jlramos{at}eez.csic.es.

Journal of Bacteriology, June 2004, p. 3439-3446, Vol. 186, No. 11
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.11.3439-3446.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Revelles, O., Wittich, R.-M., Ramos, J. L. (2007). Identification of the Initial Steps in D-Lysine Catabolism in Pseudomonas putida. J. Bacteriol. 189: 2787-2792 [Abstract] [Full Text]  
  • Munoz-Rojas, J., Bernal, P., Duque, E., Godoy, P., Segura, A., Ramos, J.-L. (2006). Involvement of Cyclopropane Fatty Acids in the Response of Pseudomonas putida KT2440 to Freeze-Drying. Appl. Environ. Microbiol. 72: 472-477 [Abstract] [Full Text]  
  • Revelles, O., Espinosa-Urgel, M., Fuhrer, T., Sauer, U., Ramos, J. L. (2005). Multiple and Interconnected Pathways for L-Lysine Catabolism in Pseudomonas putida KT2440. J. Bacteriol. 187: 7500-7510 [Abstract] [Full Text]  


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