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Journal of Bacteriology, June 2004, p. 4030-4033, Vol. 186, No. 12
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.12.4030-4033.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Acidic Nature of the CcmG Redox-Active Center Is Important for Cytochrome c Maturation in Escherichia coli

Melissa A. Edeling,^1, Umesh Ahuja,² Begoña Heras,¹ Linda Thöny-Meyer,² and Jennifer L. Martin^1*

Institute for Molecular Bioscience and Special Research Centre for Functional and Applied Genomics, The University of Queensland, Brisbane, Queensland 4072, Australia,¹ Institute for Microbiology, ETH Zürich, CH-8092 Zürich, Switzerland²

Received 20 November 2003/ Accepted 19 February 2004

Cytochrome c biogenesis in Escherichia coli is a complex process requiring at least eight genes (ccmABCDEFGH). One of these genes, ccmG, encodes a thioredoxin-like protein with unusually specific redox activity. Here, we investigate the basis for CcmG function and demonstrate the importance of acidic residues surrounding the redox-active center.

Present address: Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Cambridge CB2 2XY, United Kingdom.

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