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Journal of Bacteriology, June 2004, p. 4042-4045, Vol. 186, No. 12
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.12.4042-4045.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

MacA, a Diheme c-Type Cytochrome Involved in Fe(III) Reduction by Geobacter sulfurreducens

Jessica E. Butler, Franz Kaufmann, Maddalena V. Coppi, Cinthia Núñez, and Derek R. Lovley^*

Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003

Received 17 October 2003/ Accepted 29 February 2004

A 36-kDa diheme c-type cytochrome abundant in Fe(III)-respiring Geobacter sulfurreducens, designated MacA, was more highly expressed during growth with Fe(III) as the electron acceptor than with fumarate. Although MacA has homology to proteins with in vitro peroxidase activity, deletion of macA had no impact on response to oxidative stress. However, the capacity for Fe(III) reduction was greatly diminished, indicating that MacA, which is predicted to be localized in the periplasm, is a key intermediate in electron transfer to Fe(III).

Present address: Greenovation Biotech, Freiburg, Germany.

Present address: Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.

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