This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Butler, J. E.
Right arrow Articles by Lovley, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Butler, J. E.
Right arrow Articles by Lovley, D. R.

 Previous Article  |  Next Article 

Journal of Bacteriology, June 2004, p. 4042-4045, Vol. 186, No. 12
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.12.4042-4045.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

MacA, a Diheme c-Type Cytochrome Involved in Fe(III) Reduction by Geobacter sulfurreducens

Jessica E. Butler, Franz Kaufmann,{dagger} Maddalena V. Coppi, Cinthia Núñez,{ddagger} and Derek R. Lovley*

Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003

Received 17 October 2003/ Accepted 29 February 2004

A 36-kDa diheme c-type cytochrome abundant in Fe(III)-respiring Geobacter sulfurreducens, designated MacA, was more highly expressed during growth with Fe(III) as the electron acceptor than with fumarate. Although MacA has homology to proteins with in vitro peroxidase activity, deletion of macA had no impact on response to oxidative stress. However, the capacity for Fe(III) reduction was greatly diminished, indicating that MacA, which is predicted to be localized in the periplasm, is a key intermediate in electron transfer to Fe(III).

* Corresponding author. Mailing address: Department of Microbiology, 203 Morrill Science Center IVN, University of Massachusetts Amherst, Amherst, MA 01003. Phone: (413) 545-2735. Fax: (413) 545-1578. E-mail: dlovley{at}microbio.umass.edu.

{dagger} Present address: Greenovation Biotech, Freiburg, Germany.

{ddagger} Present address: Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.

Journal of Bacteriology, June 2004, p. 4042-4045, Vol. 186, No. 12
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.12.4042-4045.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rollefson, J. B., Levar, C. E., Bond, D. R. (2009). Identification of Genes Involved in Biofilm Formation and Respiration via Mini-Himar Transposon Mutagenesis of Geobacter sulfurreducens. J. Bacteriol. 191: 4207-4217 [Abstract] [Full Text]  
  • Law, N., Ansari, S., Livens, F. R., Renshaw, J. C., Lloyd, J. R. (2008). Formation of Nanoscale Elemental Silver Particles via Enzymatic Reduction by Geobacter sulfurreducens. Appl. Environ. Microbiol. 74: 7090-7093 [Abstract] [Full Text]  
  • Haveman, S. A., DiDonato, R. J. Jr., Villanueva, L., Shelobolina, E. S., Postier, B. L., Xu, B., Liu, A., Lovley, D. R. (2008). Genome-Wide Gene Expression Patterns and Growth Requirements Suggest that Pelobacter carbinolicus Reduces Fe(III) Indirectly via Sulfide Production. Appl. Environ. Microbiol. 74: 4277-4284 [Abstract] [Full Text]  
  • Holmes, D. E., Mester, T., O'Neil, R. A., Perpetua, L. A., Larrahondo, M. J., Glaven, R., Sharma, M. L., Ward, J. E., Nevin, K. P., Lovley, D. R. (2008). Genes for two multicopper proteins required for Fe(III) oxide reduction in Geobacter sulfurreducens have different expression patterns both in the subsurface and on energy-harvesting electrodes. Microbiology 154: 1422-1435 [Abstract] [Full Text]  
  • Coppi, M. V., O'Neil, R. A., Leang, C., Kaufmann, F., Methe, B. A., Nevin, K. P., Woodard, T. L., Liu, A., Lovley, D. R. (2007). Involvement of Geobacter sulfurreducens SfrAB in acetate metabolism rather than intracellular, respiration-linked Fe(III) citrate reduction. Microbiology 153: 3572-3585 [Abstract] [Full Text]  
  • Dale, J. R., Wade, R. Jr., DiChristina, T. J. (2007). A Conserved Histidine in Cytochrome c Maturation Permease CcmB of Shewanella putrefaciens Is Required for Anaerobic Growth below a Threshold Standard Redox Potential. J. Bacteriol. 189: 1036-1043 [Abstract] [Full Text]  
  • DiDonato, L. N., Sullivan, S. A., Methe, B. A., Nevin, K. P., England, R., Lovley, D. R. (2006). Role of RelGsu in Stress Response and Fe(III) Reduction in Geobacter sulfurreducens. J. Bacteriol. 188: 8469-8478 [Abstract] [Full Text]  
  • Haveman, S. A., Holmes, D. E., Ding, Y.-H. R., Ward, J. E., DiDonato, R. J. Jr., Lovley, D. R. (2006). c-Type Cytochromes in Pelobacter carbinolicus. Appl. Environ. Microbiol. 72: 6980-6985 [Abstract] [Full Text]  
  • Nunez, C., Esteve-Nunez, A., Giometti, C., Tollaksen, S., Khare, T., Lin, W., Lovley, D. R., Methe, B. A. (2006). DNA Microarray and Proteomic Analyses of the RpoS Regulon in Geobacter sulfurreducens.. J. Bacteriol. 188: 2792-2800 [Abstract] [Full Text]  
  • Kim, B.-C., Qian, X., Leang, C., Coppi, M. V., Lovley, D. R. (2006). Two Putative c-Type Multiheme Cytochromes Required for the Expression of OmcB, an Outer Membrane Protein Essential for Optimal Fe(III) Reduction in Geobacter sulfurreducens.. J. Bacteriol. 188: 3138-3142 [Abstract] [Full Text]  
  • Feinberg, L. F., Holden, J. F. (2006). Characterization of Dissimilatory Fe(III) versus NO3- Reduction in the Hyperthermophilic Archaeon Pyrobaculum aerophilum. J. Bacteriol. 188: 525-531 [Abstract] [Full Text]  
  • Mehta, T., Coppi, M. V., Childers, S. E., Lovley, D. R. (2005). Outer Membrane c-Type Cytochromes Required for Fe(III) and Mn(IV) Oxide Reduction in Geobacter sulfurreducens. Appl. Environ. Microbiol. 71: 8634-8641 [Abstract] [Full Text]  
  • Leang, C., Adams, L. A., Chin, K.-J., Nevin, K. P., Methe, B. A., Webster, J., Sharma, M. L., Lovley, D. R. (2005). Adaptation to Disruption of the Electron Transfer Pathway for Fe(III) Reduction in Geobacter sulfurreducens. J. Bacteriol. 187: 5918-5926 [Abstract] [Full Text]  
  • Methe, B. A., Webster, J., Nevin, K., Butler, J., Lovley, D. R. (2005). DNA Microarray Analysis of Nitrogen Fixation and Fe(III) Reduction in Geobacter sulfurreducens. Appl. Environ. Microbiol. 71: 2530-2538 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»