This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nair, S. Articles by Finkel, S. E. Search for Related Content PubMed PubMed Citation Articles by Nair, S. Articles by Finkel, S. E.

Dps Protects Cells against Multiple Stresses during Stationary Phase

Molecular and Computational Biology Program, Department of Biological Sciences, University of Southern California, Los Angeles, California 90089-1340

Received 2 December 2003/ Accepted 26 March 2004

Dps, the nonspecific DNA-binding protein from starved cells, is the most abundant protein in stationary-phase Escherichia coli. Dps homologs are found throughout the bacteria and in at least one archaeal species. Dps has been shown to protect cells from oxidative stress during exponential-phase growth. During stationary phase, Dps organizes the chromosome into a highly ordered, stable nucleoprotein complex called the biocrystal. We show here that Dps is required for long-term stationary-phase viability under competitive conditions and that dps mutants have altered lag phases compared to wild-type cells. We also show that during stationary phase Dps protects the cell not only from oxidative stress but also from UV and gamma irradiation, iron and copper toxicity, thermal stress, and acid and base shock. The protective roles of Dps are most likely achieved through a combination of functions associated with the protein-DNA binding and chromosome compaction, metal chelation, ferroxidase activity, and regulation of gene expression.

This article has been cited by other articles:

• LeBlanc, J. C., Goncalves, E. R., Mohn, W. W. (2008). Global Response to Desiccation Stress in the Soil Actinomycete Rhodococcus jostii RHA1. Appl. Environ. Microbiol. 74: 2627-2636 [Abstract] [Full Text]  
• Whitehead, K., Versalovic, J., Roos, S., Britton, R. A. (2008). Genomic and Genetic Characterization of the Bile Stress Response of Probiotic Lactobacillus reuteri ATCC 55730. Appl. Environ. Microbiol. 74: 1812-1819 [Abstract] [Full Text]  
• Bagwell, C. E., Milliken, C. E., Ghoshroy, S., Blom, D. A. (2008). Intracellular Copper Accumulation Enhances the Growth of Kineococcus radiotolerans during Chronic Irradiation. Appl. Environ. Microbiol. 74: 1376-1384 [Abstract] [Full Text]  
• Gourion, B., Francez-Charlot, A., Vorholt, J. A. (2008). PhyR Is Involved in the General Stress Response of Methylobacterium extorquens AM1. J. Bacteriol. 190: 1027-1035 [Abstract] [Full Text]  
• Abdallah, J., Caldas, T., Kthiri, F., Kern, R., Richarme, G. (2007). YhbO Protects Cells against Multiple Stresses. J. Bacteriol. 189: 9140-9144 [Abstract] [Full Text]  
• Saumaa, S., Tover, A., Tark, M., Tegova, R., Kivisaar, M. (2007). Oxidative DNA Damage Defense Systems in Avoidance of Stationary-Phase Mutagenesis in Pseudomonas putida. J. Bacteriol. 189: 5504-5514 [Abstract] [Full Text]  
• Bore, E., Hebraud, M., Chafsey, I., Chambon, C., Skjaeret, C., Moen, B., Moretro, T., Langsrud, O., Rudi, K., Langsrud, S. (2007). Adapted tolerance to benzalkonium chloride in Escherichia coli K-12 studied by transcriptome and proteome analyses. Microbiology 153: 935-946 [Abstract] [Full Text]  
• Ceci, P., Mangiarotti, L., Rivetti, C., Chiancone, E. (2007). The neutrophil-activating Dps protein of Helicobacter pylori, HP-NAP, adopts a mechanism different from Escherichia coli Dps to bind and condense DNA. Nucleic Acids Res 35: 2247-2256 [Abstract] [Full Text]  
• Liu, X., Kim, K., Leighton, T., Theil, E. C. (2006). Paired Bacillus anthracis Dps (Mini-ferritin) Have Different Reactivities with Peroxide. J. Biol. Chem. 281: 27827-27835 [Abstract] [Full Text]  
• Gourion, B., Rossignol, M., Vorholt, J. A. (2006). A proteomic study of Methylobacterium extorquens reveals a response regulator essential for epiphytic growth. Proc. Natl. Acad. Sci. USA 103: 13186-13191 [Abstract] [Full Text]  
• Junker, L. M., Peters, J. E., Hay, A. G. (2006). Global analysis of candidate genes important for fitness in a competitive biofilm using DNA-array-based transposon mapping.. Microbiology 152: 2233-2245 [Abstract] [Full Text]  
• Kaur, A., Pan, M., Meislin, M., Facciotti, M. T., El-Gewely, R., Baliga, N. S. (2006). A systems view of haloarchaeal strategies to withstand stress from transition metals. Genome Res. 16: 841-854 [Abstract] [Full Text]  
• Castruita, M., Saito, M., Schottel, P. C., Elmegreen, L. A., Myneni, S., Stiefel, E. I., Morel, F. M. M. (2006). Overexpression and Characterization of an Iron Storage and DNA-Binding Dps Protein from Trichodesmium erythraeum. Appl. Environ. Microbiol. 72: 2918-2924 [Abstract] [Full Text]  
• Morikawa, K., Ohniwa, R. L., Kim, J., Maruyama, A., Ohta, T., Takeyasu, K. (2006). Bacterial nucleoid dynamics: oxidative stress response in Staphylococcus aureus. GENES CELLS 11: 409-423 [Abstract] [Full Text]  
• Brombacher, E., Baratto, A., Dorel, C., Landini, P. (2006). Gene Expression Regulation by the Curli Activator CsgD Protein: Modulation of Cellulose Biosynthesis and Control of Negative Determinants for Microbial Adhesion. J. Bacteriol. 188: 2027-2037 [Abstract] [Full Text]  
• Johnson, M. R., Conners, S. B., Montero, C. I., Chou, C. J., Shockley, K. R., Kelly, R. M. (2006). The Thermotoga maritima Phenotype Is Impacted by Syntrophic Interaction with Methanococcus jannaschii in Hyperthermophilic Coculture. Appl. Environ. Microbiol. 72: 811-818 [Abstract] [Full Text]  
• Lacqua, A., Wanner, O., Colangelo, T., Martinotti, M. G., Landini, P. (2006). Emergence of Biofilm-Forming Subpopulations upon Exposure of Escherichia coli to Environmental Bacteriophages. Appl. Environ. Microbiol. 72: 956-959 [Abstract] [Full Text]  
• Flint, J. F., Drzymalski, D., Montgomery, W. L., Southam, G., Angert, E. R. (2005). Nocturnal Production of Endospores in Natural Populations of Epulopiscium-Like Surgeonfish Symbionts. J. Bacteriol. 187: 7460-7470 [Abstract] [Full Text]  
• Kao, K. C., Tran, L. M., Liao, J. C. (2005). A Global Regulatory Role of Gluconeogenic Genes in Escherichia coli Revealed by Transcriptome Network Analysis. J. Biol. Chem. 280: 36079-36087 [Abstract] [Full Text]  
• Ceci, P., Ilari, A., Falvo, E., Giangiacomo, L., Chiancone, E. (2005). Reassessment of Protein Stability, DNA Binding, and Protection of Mycobacterium smegmatis Dps. J. Biol. Chem. 280: 34776-34785 [Abstract] [Full Text]  
• Wiedenheft, B., Mosolf, J., Willits, D., Yeager, M., Dryden, K. A., Young, M., Douglas, T. (2005). From The Cover: An archaeal antioxidant: Characterization of a Dps-like protein from Sulfolobus solfataricus. Proc. Natl. Acad. Sci. USA 102: 10551-10556 [Abstract] [Full Text]  
• Park, S., You, X., Imlay, J. A. (2005). Substantial DNA damage from submicromolar intracellular hydrogen peroxide detected in Hpx- mutants of Escherichia coli. Proc. Natl. Acad. Sci. USA 102: 9317-9322 [Abstract] [Full Text]  
• Olsen, K. N., Larsen, M. H., Gahan, C. G. M., Kallipolitis, B., Wolf, X. A., Rea, R., Hill, C., Ingmer, H. (2005). The Dps-like protein Fri of Listeria monocytogenes promotes stress tolerance and intracellular multiplication in macrophage-like cells. Microbiology 151: 925-933 [Abstract] [Full Text]  
• Ceci, P., Cellai, S., Falvo, E., Rivetti, C., Rossi, G. L., Chiancone, E. (2004). DNA condensation and self-aggregation of Escherichia coli Dps are coupled phenomena related to the properties of the N-terminus. Nucleic Acids Res 32: 5935-5944 [Abstract] [Full Text]