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tonB3 Is Required for Normal Twitching Motility and Extracellular Assembly of Type IV Pili

Bixing Huang, Kelin Ru, Zheng Yuan, Cynthia B. Whitchurch, and John S. Mattick^*

Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia

Received 26 February 2004/ Accepted 29 March 2004

Three mutants with Tn5-B21 insertion in tonB3 (PA0406) of Pseudomonas aeruginosa exhibited defective twitching motility and reduced assembly of extracellular pili. These defects could be complemented with wild-type tonB3.

Present address: Public Health Microbiology Laboratory, Queensland Health Scientific Services, Coopers Plains, Q 4108, Australia.

Present address: Department of Microbiology, Monash University, Clayton, VIC 3168, Australia.

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