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Journal of Bacteriology, July 2004, p. 4399-4401, Vol. 186, No. 13
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.13.4399-4401.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Independent Regulation of Two Genes in Escherichia coli by Tetracyclines and Tet Repressor Variants

Lehrstuhl für Mikrobiologie, Biochemie und Genetik, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91058 Erlangen, Germany

Received 4 February 2004/ Accepted 3 April 2004

We report a regulation system in Escherichia coli for independent regulation of two distinct reporter genes by application of Tet repressors with different specificities. One Tet repressor variant comprises wild-type tet operator (tetO) recognition and exclusive induction with the novel inducer 4-dedimethylamino-anhydrotetracycline. The other Tet repressor variant shows tetO-4C recognition and induction with tetracycline. We demonstrate that both variants are independently active in vivo and allow selective regulation of two genes in the same cell without any cross talk.

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