This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by White, A. K.
Right arrow Articles by Metcalf, W. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by White, A. K.
Right arrow Articles by Metcalf, W. W.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 2004, p. 4730-4739, Vol. 186, No. 14
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.14.4730-4739.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Two C—P Lyase Operons in Pseudomonas stutzeri and Their Roles in the Oxidation of Phosphonates, Phosphite, and Hypophosphite

Andrea K. White and William W. Metcalf*

Chemical and Life Sciences Laboratory, Department of Microbiology, University of Illinois, Urbana, Illinois 61801

Received 30 January 2004/ Accepted 5 April 2004

DNA sequencing and analysis of two distinct CP lyase operons in Pseudomonas stutzeri WM88 were completed. The htxABCDEFGHIJKLMN operon encodes a hypophosphite-2-oxoglutarate dioxygenase (HtxA), whereas the predicted amino acid sequences of HtxB to HtxN are each homologous to the components of the Escherichia coli phn operon, which encodes CP lyase, although homologs of E. coli phnF and phnO are absent. The genes in the htx operon are cotranscribed based on gene organization, and the presence of the intergenic sequences is verified by reverse transcription-PCR with total RNA. Deletion of the htx locus does not affect the ability of P. stutzeri to grow on phosphonates, indicating the presence of an additional CP lyase pathway in this organism. To identify the genes comprising this pathway, a {Delta}htx strain was mutagenized and one mutant lacking the ability to grow on methylphosphonate as the sole P source was isolated. A ca.-10.6-kbp region surrounding the transposon insertion site of this mutant was sequenced, revealing 13 open reading frames, designated phnCDEFGHIJKLMNP, which were homologous to the E. coli phn genes. Deletion of both the htx and phn operons of P. stutzeri abolishes all growth on methylphosphonate and aminoethylphosphonate. Both operons individually support growth on methylphosphonate; however, the phn operon supports growth on aminoethylphosphonate and phosphite, as well. The substrate ranges of both CP lyases are limited, as growth on other phosphonate compounds, including glyphosate and phenylphosphonate, was not observed.

* Corresponding author. Mailing address: Department of Microbiology, University of Illinois, B103 Chemical and Life Sciences Laboratory, 601 S. Goodwin Ave., Urbana, IL 61801. Phone: (217) 244-1943. Fax: (217) 244-6697. E-mail: metcalf{at}uiuc.edu.

Journal of Bacteriology, July 2004, p. 4730-4739, Vol. 186, No. 14
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.14.4730-4739.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Adams, M. M., Gomez-Garcia, M. R., Grossman, A. R., Bhaya, D. (2008). Phosphorus Deprivation Responses and Phosphonate Utilization in a Thermophilic Synechococcus sp. from Microbial Mats. J. Bacteriol. 190: 8171-8184 [Abstract] [Full Text]  
  • Gebhard, S., Cook, G. M. (2008). Differential Regulation of High-Affinity Phosphate Transport Systems of Mycobacterium smegmatis: Identification of PhnF, a Repressor of the phnDCE Operon. J. Bacteriol. 190: 1335-1343 [Abstract] [Full Text]  
  • Adams, M. A., Luo, Y., Hove-Jensen, B., He, S.-M., van Staalduinen, L. M., Zechel, D. L., Jia, Z. (2008). Crystal Structure of PhnH: an Essential Component of Carbon-Phosphorus Lyase in Escherichia coli. J. Bacteriol. 190: 1072-1083 [Abstract] [Full Text]  
  • Pasek, M. A. (2008). Rethinking early Earth phosphorus geochemistry. Proc. Natl. Acad. Sci. USA 105: 853-858 [Abstract] [Full Text]  
  • Kane, S. R., Chakicherla, A. Y., Chain, P. S. G., Schmidt, R., Shin, M. W., Legler, T. C., Scow, K. M., Larimer, F. W., Lucas, S. M., Richardson, P. M., Hristova, K. R. (2007). Whole-Genome Analysis of the Methyl tert-Butyl Ether-Degrading Beta-Proteobacterium Methylibium petroleiphilum PM1. J. Bacteriol. 189: 1931-1945 [Abstract] [Full Text]  
  • Gebhard, S., Tran, S. L., Cook, G. M. (2006). The Phn system of Mycobacterium smegmatis: a second high-affinity ABC-transporter for phosphate.. Microbiology 152: 3453-3465 [Abstract] [Full Text]  
  • Monds, R. D., Newell, P. D., Schwartzman, J. A., O'Toole, G. A. (2006). Conservation of the Pho regulon in Pseudomonas fluorescens Pf0-1.. Appl. Environ. Microbiol. 72: 1910-1924 [Abstract] [Full Text]  
  • Dyhrman, S. T., Haley, S. T. (2006). Phosphorus Scavenging in the Unicellular Marine Diazotroph Crocosphaera watsonii. Appl. Environ. Microbiol. 72: 1452-1458 [Abstract] [Full Text]  
  • Wilson, M. M., Metcalf, W. W. (2005). Genetic Diversity and Horizontal Transfer of Genes Involved in Oxidation of Reduced Phosphorus Compounds by Alcaligenes faecalis WM2072. Appl. Environ. Microbiol. 71: 290-296 [Abstract] [Full Text]  
  • White, A. K., Metcalf, W. W. (2004). The htx and ptx Operons of Pseudomonas stutzeri WM88 Are New Members of the Pho Regulon. J. Bacteriol. 186: 5876-5882 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»