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Journal of Bacteriology, July 2004, p. 4802-4807, Vol. 186, No. 14
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.14.4802-4807.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Role of Escherichia coli DNA Polymerase IV in In Vivo Replication Fidelity

Wojciech Kuban,1 Piotr Jonczyk,1 Damian Gawel,1,2 Karolina Malanowska,1 Roel M. Schaaper,2 and Iwona J. Fijalkowska1*

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02 106 Warsaw, Poland,1 Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 277092

Received 31 January 2004/ Accepted 7 April 2004

We have investigated whether DNA polymerase IV (Pol IV; the dinB gene product) contributes to the error rate of chromosomal DNA replication in Escherichia coli. We compared mutation frequencies in mismatch repair-defective strains that were either dinB positive or dinB deficient, using a series of mutational markers, including lac targets in both orientations on the chromosome. Virtually no contribution of Pol IV to the chromosomal mutation rate was observed. On the other hand, a significant effect of dinB was observed for reversion of a lac allele when the lac gene resided on an F'(pro-lac) episome.

* Corresponding author. Mailing address: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego. 5A, 02106 Warsaw, Poland. Phone: (48) 22-6584734. Fax: (48) 39-121623. E-mail: iwonaf{at}ibb.waw.pl.

Journal of Bacteriology, July 2004, p. 4802-4807, Vol. 186, No. 14
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.14.4802-4807.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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