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Role of Escherichia coli DNA Polymerase IV in In Vivo Replication Fidelity

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02 106 Warsaw, Poland,¹ Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709²

Received 31 January 2004/ Accepted 7 April 2004

We have investigated whether DNA polymerase IV (Pol IV; the dinB gene product) contributes to the error rate of chromosomal DNA replication in Escherichia coli. We compared mutation frequencies in mismatch repair-defective strains that were either dinB positive or dinB deficient, using a series of mutational markers, including lac targets in both orientations on the chromosome. Virtually no contribution of Pol IV to the chromosomal mutation rate was observed. On the other hand, a significant effect of dinB was observed for reversion of a lac allele when the lac gene resided on an F'(pro-lac) episome.

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