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Journal of Bacteriology, July 2004, p. 4829-4833, Vol. 186, No. 14
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.14.4829-4833.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Reverse Gyrase Is Not a Prerequisite for Hyperthermophilic Life

Haruyuki Atomi,1,2 Rie Matsumi,1 and Tadayuki Imanaka1,2*

Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering,1 Katsura Int'tech Center, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan2

Received 12 December 2003/ Accepted 14 April 2004

We disrupted the reverse gyrase gene from a hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1. An apparent positive supercoiling activity that was observed in the host strain was not found in the disruptant strain. We found that a lack of reverse gyrase led to a retardation in growth that was more striking at higher temperatures. However, the disruption of the reverse gyrase gene did not lead to a lethal phenotype at 90°C. This study provides experimental evidence that reverse gyrase is not a prerequisite for hyperthermophilic life.

* Corresponding author. Mailing address: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. Phone: 81-75-383-2777. Fax: 81-75-383-2778. E-mail: imanaka{at}sbchem.kyoto-u.ac.jp.

Journal of Bacteriology, July 2004, p. 4829-4833, Vol. 186, No. 14
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.14.4829-4833.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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