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Three Different Systems Participate in L-Cystine Uptake in Bacillus subtilis

Pierre Burguière, Sandrine Auger, Marie-Françoise Hullo, Antoine Danchin, and Isabelle Martin-Verstraete^*

Unité de Génétique des Génomes Bactériens, Institut Pasteur, URA CNRS 2171, 75724 Paris Cedex 15, France

Received 19 February 2004/ Accepted 4 May 2004

The symporter YhcL and two ATP binding cassette transporters, YtmJKLMN and YckKJI, were shown to mediate L-cystine uptake in Bacillus subtilis. A triple yhcL ytmJKLMN yckK mutant was unable to grow in the presence of L-cystine and to take up L-cystine. We propose that yhcL, ytmJKLMN, and yckKJI should be renamed tcyP, tcyJKLMN, and tcyABC, respectively. The L-cystine uptake by YhcL (K_m = 0.6 µM) was strongly inhibited by seleno-DL-cystine, while the transport due to the YtmJKLMN system (K_m = 2.5 µM) also drastically decreased in the presence of DL-cystathionine, L-djenkolic acid, or S-methyl-L-cysteine. Accordingly, a ytmJKLMN mutant did not grow in the presence of 100 µM DL-cystathionine, 100 µM L-djenkolic acid, or 100 µM S-methyl-L-cysteine. The expression of the ytmI operon and the yhcL gene was regulated in response to sulfur availability, while the level of expression of the yckK gene remained low under all the conditions tested.

Present address: Laboratoire de Microbiologie et Génétique Moléculaire, INRA-CNRS URA1925, 78850 Thiverval-Grignon, France.

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