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Journal of Bacteriology, August 2004, p. 4972-4977, Vol. 186, No. 15
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.15.4972-4977.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Circadian Rhythms in the Thermophilic Cyanobacterium Thermosynechococcus elongatus: Compensation of Period Length over a Wide Temperature Range

Kiyoshi Onai,1,2,{dagger} Megumi Morishita,1,{dagger} Shino Itoh,1,3 Kazuhisa Okamoto,1,4 and Masahiro Ishiura1,2,3,4*

Center for Gene Research,1 Bio-Oriented Technology Research Advancement Institution,2 Division of Biological Science, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602,3 Aichi Science and Technology Foundation, Naka, Nagoya 460-0002, Japan4

Received 2 February 2004/ Accepted 5 May 2004

Proteins derived from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1, which performs plant-type oxygenic photosynthesis, are suitable for biochemical, biophysical, and X-ray crystallographic studies. We developed an automated bioluminescence real-time monitoring system for the circadian clock in the thermophilic cyanobacterium T. elongatus BP-1 that uses a bacterial luciferase gene set (Xl luxAB) derived from Xenorhabdus luminescens as a bioluminescence reporter gene. A promoter region of the psbA1 gene of T. elongatus was fused to the Xl luxAB gene set and inserted into a specific targeting site in the genome of T. elongatus. The bioluminescence from the cells of the psbA1-reporting strain was measured by an automated monitoring apparatus with photomultiplier tubes. The strain exhibited the circadian rhythms of bioluminescence with a 25-h period length for at least 10 days in constant light and temperature. The rhythms were reset by light-dark cycle, and their period length was almost constant over a wide range of temperatures (30 to 60°C). Theses results indicate that T. elongatus has the circadian clock that is widely temperature compensated.

* Corresponding author. Mailing address: Center for Gene Research, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan. Phone: 81-52-789-4527. Fax: 81-52-789-4526. E-mail: ishiura{at}gene.nagoya-u.ac.jp.

{dagger} K.O. and M.M. contributed equally to this study.

Journal of Bacteriology, August 2004, p. 4972-4977, Vol. 186, No. 15
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.15.4972-4977.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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