Previous Article | Next Article 
Journal of Bacteriology, August 2004, p. 4994-5002, Vol. 186, No. 15
0021-9193/04/$08.00+0 DOI: 10.1128/JB.186.15.4994-5002.2004
Intraspecific Phylogeny and Lineage Group Identification Based on the prfA Virulence Gene Cluster of Listeria monocytogenes
Todd J. Ward,1* Lisa Gorski,2 Monica K. Borucki,3 Robert E. Mandrell,2 Jan Hutchins,4 and Kitty Pupedis4
Microbial
Genomics and Bioprocessing Research Unit, Agricultural Research
Service, U.S. Department of Agriculture, Peoria, Illinois
61604,1
Produce Safety and
Microbiology Research Unit, Agricultural Research Service, U.S.
Department of Agriculture, Albany, California
94710,2
Animal Disease Research Unit,
Agricultural Research Service, U.S. Department of Agriculture,
Pullman, Washington 99164,3
Microbial Outbreaks and
Special Projects Laboratory, Food Safety and Inspection
Service, U.S. Department of Agriculture, Athens, Georgia
306054
Received 15 December 2003/
Accepted 23 April 2004
Listeria
monocytogenes is a serious food-borne pathogen that can cause
invasive disease in humans and other animals and has been the leading
cause of food recalls due to microbiological concerns in recent years.
In order to test hypotheses regarding L.
monocytogenes lineage composition, evolution, ecology, and
taxonomy, a robust intraspecific phylogeny was developed based on
prfA virulence gene cluster sequences from 113 L.
monocytogenes isolates. The results of the multigene
phylogenetic analyses confirm that L. monocytogenes
comprises at least three evolutionary lineages, demonstrate that
lineages most frequently (lineage 1) and least frequently (lineage 3)
associated with human listeriosis are sister-groups, and reveal for the
first time that the human epidemic associated serotype 4b is prevalent
among strains from lineage 1 and lineage 3. In addition, a PCR-based
test for lineage identification was developed and used in a survey of
food products demonstrating that the low frequency of association
between lineage 3 isolates and human listeriosis cases likely reflects
rarity of exposure and not reduced virulence for humans as has been
previously suggested. However, prevalence data do suggest lineage 3
isolates may be better adapted to the animal production environment
than the food-processing environment. Finally, analyses of haplotype
diversity indicate that lineage 1 has experienced a purge of genetic
variation that was not observed in the other lineages, suggesting that
the three L. monocytogenes lineages may represent
distinct species within the framework of the cohesion species
concept.
* Corresponding
author. Mailing address: Microbial Genomics and
Bioprocessing Research Unit, National Center for Agricultural
Utilization Research, Agricultural Research Service, U.S. Department of
Agriculture, 1815 North University St., Peoria, IL 61604. Phone: (309)
681-6394. Fax: (309) 681-6672. E-mail:
wardtj{at}ncaur.usda.gov.
Supplemental material for this article may be found at http://jb.asm.org/.
Journal of Bacteriology, August 2004, p. 4994-5002, Vol. 186, No. 15
0021-9193/04/$08.00+0 DOI: 10.1128/JB.186.15.4994-5002.2004
This article has been cited by other articles:
-
Severino, P., Dussurget, O., Vencio, R. Z. N., Dumas, E., Garrido, P., Padilla, G., Piveteau, P., Lemaitre, J.-P., Kunst, F., Glaser, P., Buchrieser, C.
(2007). Comparative Transcriptome Analysis of Listeria monocytogenes Strains of the Two Major Lineages Reveals Differences in Virulence, Cell Wall, and Stress Response. Appl. Environ. Microbiol.
73: 6078-6088
[Abstract]
[Full Text]
-
Orsi, R. H., Ripoll, D. R., Yeung, M., Nightingale, K. K., Wiedmann, M.
(2007). Recombination and positive selection contribute to evolution of Listeria monocytogenes inlA. Microbiology
153: 2666-2678
[Abstract]
[Full Text]
-
Volokhov, D. V., Duperrier, S., Neverov, A. A., George, J., Buchrieser, C., Hitchins, A. D.
(2007). The Presence of the Internalin Gene in Natural Atypically Hemolytic Listeria innocua Strains Suggests Descent from L. monocytogenes. Appl. Environ. Microbiol.
73: 1928-1939
[Abstract]
[Full Text]
-
Fugett, E. B., Schoonmaker-Bopp, D., Dumas, N. B., Corby, J., Wiedmann, M.
(2007). Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Temporally Matched Listeria monocytogenes Isolates from Human Clinical Cases, Foods, Ruminant Farms, and Urban and Natural Environments Reveals Source-Associated as Well as Widely Distributed PFGE Types. J. Clin. Microbiol.
45: 865-873
[Abstract]
[Full Text]
-
Chen, Y., Zhang, W., Knabel, S. J.
(2007). Multi-Virulence-Locus Sequence Typing Identifies Single Nucleotide Polymorphisms Which Differentiate Epidemic Clones and Outbreak Strains of Listeria monocytogenes. J. Clin. Microbiol.
45: 835-846
[Abstract]
[Full Text]
-
Ducey, T. F., Page, B., Usgaard, T., Borucki, M. K., Pupedis, K., Ward, T. J.
(2007). A Single-Nucleotide-Polymorphism-Based Multilocus Genotyping Assay for Subtyping Lineage I Isolates of Listeria monocytogenes. Appl. Environ. Microbiol.
73: 133-147
[Abstract]
[Full Text]
-
Liu, D.
(2006). Identification, subtyping and virulence determination of Listeria monocytogenes, an important foodborne pathogen. J Med Microbiol
55: 645-659
[Abstract]
[Full Text]
-
Rooney, A. P., Swezey, J. L., Friedman, R., Hecht, D. W., Maddox, C. W.
(2006). Analysis of Core Housekeeping and Virulence Genes Reveals Cryptic Lineages of Clostridium perfringens That Are Associated With Distinct Disease Presentations. Genetics
172: 2081-2092
[Abstract]
[Full Text]
-
Roberts, A., Nightingale, K., Jeffers, G., Fortes, E., Kongo, J. M., Wiedmann, M.
(2006). Genetic and phenotypic characterization of Listeria monocytogenes lineage III.. Microbiology
152: 685-693
[Abstract]
[Full Text]
-
Gorski, L., Flaherty, D., Mandrell, R. E.
(2006). Competitive Fitness of Listeria monocytogenes Serotype 1/2a and 4b Strains in Mixed Cultures with and without Food in the U.S. Food and Drug Administration Enrichment Protocol. Appl. Environ. Microbiol.
72: 776-783
[Abstract]
[Full Text]
-
Liu, D., Lawrence, M. L., Gorski, L., Mandrell, R. E., Ainsworth, A. J., Austin, F. W.
(2006). Listeria monocytogenes Serotype 4b Strains Belonging to Lineages I and III Possess Distinct Molecular Features. J. Clin. Microbiol.
44: 214-217
[Abstract]
[Full Text]
-
Gilbreth, S. E., Call, J. E., Wallace, F. M., Scott, V. N., Chen, Y., Luchansky, J. B.
(2005). Relatedness of Listeria monocytogenes Isolates Recovered from Selected Ready-To-Eat Foods and Listeriosis Patients in the United States. Appl. Environ. Microbiol.
71: 8115-8122
[Abstract]
[Full Text]
-
Faith, N., Uhlich, G., Luchansky, J. B., Neudeck, B., Czuprynski, C.
(2005). A prfA Transposon Mutant of Listeria monocytogenes F2365, a Serotype 4b Strain, Is Able To Survive in the Gastrointestinal Tract but Does Not Cause Systemic Infection of the Spleens and Livers of Intragastrically Inoculated Mice. Infect. Immun.
73: 7517-7524
[Abstract]
[Full Text]
-
Zhang, C., Nietfeldt, J., Zhang, M., Benson, A. K.
(2005). Functional Consequences of Genome Evolution in Listeria monocytogenes: the lmo0423 and lmo0422 Genes Encode {sigma}C and LstR, a Lineage II-Specific Heat Shock System. J. Bacteriol.
187: 7243-7253
[Abstract]
[Full Text]
-
Borucki, M. K., Gay, C. C., Reynolds, J., McElwain, K. L., Kim, S. H., Call, D. R., Knowles, D. P.
(2005). Genetic Diversity of Listeria monocytogenes Strains from a High-Prevalence Dairy Farm. Appl. Environ. Microbiol.
71: 5893-5899
[Abstract]
[Full Text]
-
Borucki, M. K., Reynolds, J., Call, D. R., Ward, T. J., Page, B., Kadushin, J.
(2005). Suspension Microarray with Dendrimer Signal Amplification Allows Direct and High-Throughput Subtyping of Listeria monocytogenes from Genomic DNA. J. Clin. Microbiol.
43: 3255-3259
[Abstract]
[Full Text]
-
Rooney, A. P., Ward, T. J.
(2005). Evolution of a large ribosomal RNA multigene family in filamentous fungi: Birth and death of a concerted evolution paradigm. Proc. Natl. Acad. Sci. USA
102: 5084-5089
[Abstract]
[Full Text]
Copyright © 2004 by the American Society for Microbiology. All rights reserved.