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Journal of Bacteriology, August 2004, p. 5078-5086, Vol. 186, No. 15
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.15.5078-5086.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Secretion and Function of Salmonella SPI-2 Effector SseF Require Its Chaperone, SscB

Shipan Dai and Daoguo Zhou*

Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907

Received 1 March 2004/ Accepted 5 May 2004

Salmonella strains utilize a type III secretion system for their successful survival and replications inside host cells. SseF is one of the several effector proteins that are required for conferring this survival ability by altering the trafficking of the Salmonella-containing vacuoles. These effector proteins often require appropriate chaperones to maintain their stabilities inside the bacteria. These chaperones are also known to assist the subsequent secretion and translocation of their substrates. We report here that SscB acts as the chaperone for SseF, an effector for the Salmonella pathogenicity island 2 (SPI-2). We found that the sscB gene is required for the formation of Salmonella sp.-induced continuous filaments in epithelial cells. Efficient Salmonella replication in macrophages requires SscB function. Intracellular and secretion levels of SseF are greatly reduced in an sscB mutant strain compared to the wild-type strain. A protein stability assay demonstrated that the half-life of SseF is significantly shortened in the absence of SscB. Transcriptional analysis of the sseF gene showed that the effect of SscB on the SseF level is not at the transcriptional level. A coprecipitation experiment indicated that SscB interacts with SseF. In summary, our results indicate that SscB is a chaperone for SPI-2 effector SseF to facilitate its secretion and function inside the host cells.

* Corresponding author. Mailing address: Department of Biological Sciences, Purdue University, West Lafayette, IN 47907. Phone: (765) 494-8159. Fax: (765) 494-0876. E-mail: zhoud{at}purdue.edu.

Journal of Bacteriology, August 2004, p. 5078-5086, Vol. 186, No. 15
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.15.5078-5086.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhang, Y., Higashide, W., Dai, S., Sherman, D. M., Zhou, D. (2005). Recognition and Ubiquitination of Salmonella Type III Effector SopA by a Ubiquitin E3 Ligase, HsRMA1. J. Biol. Chem. 280: 38682-38688 [Abstract] [Full Text]  
  • Suvarnapunya, A. E., Stein, M. A. (2005). DNA base excision repair potentiates the protective effect of Salmonella Pathogenicity Island 2 within macrophages. Microbiology 151: 557-567 [Abstract] [Full Text]  


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