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Journal of Bacteriology, August 2004, p. 5157-5159, Vol. 186, No. 15
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.15.5157-5159.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Cellular Levels of trp RNA-Binding Attenuation Protein in Bacillus subtilis

Barbara C. McCabe and Paul Gollnick*

Department of Biological Sciences, University at Buffalo, The State University of New York, Buffalo, New York 14260

Received 14 April 2004/ Accepted 28 April 2004

Expression of the Bacillus subtilis trp genes is negatively regulated by an 11-subunit trp RNA-binding attenuation protein (TRAP), which is activated to bind RNA by binding L-tryptophan. We used Western blotting to estimate that there are 200 to 400 TRAP 11-mer molecules per cell in cells grown in either minimal or rich medium.

* Corresponding author. Mailing address: Department of Biological Sciences, University at Buffalo, The State University of New York, Buffalo, NY 14260. Phone: (716) 645-2363, ext.189. Fax: (716) 645-2975. E-mail: Gollnick{at}acsu.buffalo.edu.

Journal of Bacteriology, August 2004, p. 5157-5159, Vol. 186, No. 15
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.15.5157-5159.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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