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Cellular Levels of trp RNA-Binding Attenuation Protein in Bacillus subtilis

Department of Biological Sciences, University at Buffalo, The State University of New York, Buffalo, New York 14260

Received 14 April 2004/ Accepted 28 April 2004

Expression of the Bacillus subtilis trp genes is negatively regulated by an 11-subunit trp RNA-binding attenuation protein (TRAP), which is activated to bind RNA by binding L-tryptophan. We used Western blotting to estimate that there are 200 to 400 TRAP 11-mer molecules per cell in cells grown in either minimal or rich medium.

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