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Journal of Bacteriology, August 2004, p. 5160-5166, Vol. 186, No. 15
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.15.5160-5166.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Expression of a Functional Secreted YopN-TyeA Hybrid Protein in Yersinia pestis Is the Result of a +1 Translational Frameshift Event

Franco Ferracci, James B. Day,^, Heather J. Ezelle, and Gregory V. Plano^*

Department of Microbiology and Immunology, University of Miami School of Medicine, Miami, Florida 33101

Received 20 February 2004/ Accepted 21 April 2004

YopN is a secreted protein that prior to secretion directly interacts with the cytosolic SycN/YscB chaperone complex and TyeA. This study identifies a secreted YopN-TyeA hybrid protein that is expressed by Yersinia pestis, but not by Yersinia enterocolitica. DNA sequence analysis and site-directed mutagenesis studies demonstrate that the hybrid protein is the result of a +1 translational frameshift event.

F.F., J.B.D., and H.E. contributed equally to this work.

Present address: Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740.

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