This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ferré, A.
Right arrow Articles by Dreyfus, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ferré, A.
Right arrow Articles by Dreyfus, G.

 Previous Article  |  Next Article 

Journal of Bacteriology, August 2004, p. 5172-5177, Vol. 186, No. 15
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.15.5172-5177.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Biochemical Study of Multiple CheY Response Regulators of the Chemotactic Pathway of Rhodobacter sphaeroides{dagger}

Axelle Ferré,1,{ddagger} Javier de la Mora,1,{ddagger} Teresa Ballado,1,{ddagger} Laura Camarena,2 and Georges Dreyfus1*

Departamento de Genética Molecular, Instituto de Fisiología Celular,1 Departamento de Biología Molecular, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 04510 Mexico DF, Mexico2

Received 3 March 2004/ Accepted 22 April 2004

The six copies of the response regulator CheY from Rhodobacter sphaeroides bind to the switch protein FliM. Phosphorylation by acetyl phosphate (AcP) was detected by tryptophan fluorescence quenching in three of the four CheYs that contain this residue. Autophosphorylation with Ac32P was observed in five CheY proteins. We also show that all of the cheY genes are expressed simultaneously; therefore, in vivo all of the CheY proteins could bind to FliM to control the chemotactic response. Consequently, we hypothesize that in this complex chemotactic system, the binding of some CheY proteins to FliM, does not necessarily imply switching of the flagellar motor.

* Corresponding author. Mailing address: Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, AP Postal 70-243, 04510 Mexico DF, Mexico. Phone: 5255-5622-5618. Fax: 5255-5616-2282. E-mail: gdreyfus{at}ifc.unam.mx.

{dagger} This study is dedicated to the memory of our dear friend Robert M. Macnab.

{ddagger} A.F., J.D.L.M., and T.B. contributed equally to this study.

Journal of Bacteriology, August 2004, p. 5172-5177, Vol. 186, No. 15
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.15.5172-5177.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Porter, S. L., Roberts, M. A. J., Manning, C. S., Armitage, J. P. (2008). A bifunctional kinase-phosphatase in bacterial chemotaxis. Proc. Natl. Acad. Sci. USA 105: 18531-18536 [Abstract] [Full Text]  
  • del Campo, A. M., Ballado, T., de la Mora, J., Poggio, S., Camarena, L., Dreyfus, G. (2007). Chemotactic Control of the Two Flagellar Systems of Rhodobacter sphaeroides Is Mediated by Different Sets of CheY and FliM Proteins. J. Bacteriol. 189: 8397-8401 [Abstract] [Full Text]  
  • Porter, S. L., Wadhams, G. H., Martin, A. C., Byles, E. D., Lancaster, D. E., Armitage, J. P. (2006). The CheYs of Rhodobacter sphaeroides. J. Biol. Chem. 281: 32694-32704 [Abstract] [Full Text]  
  • Hyakutake, A., Homma, M., Austin, M. J., Boin, M. A., Hase, C. C., Kawagishi, I. (2005). Only One of the Five CheY Homologs in Vibrio cholerae Directly Switches Flagellar Rotation. J. Bacteriol. 187: 8403-8410 [Abstract] [Full Text]  
  • Wolfe, A. J. (2005). The Acetate Switch. Microbiol. Mol. Biol. Rev. 69: 12-50 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»