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Journal of Bacteriology, August 2004, p. 5506-5512, Vol. 186, No. 16
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.16.5506-5512.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Escherichia coli Serogroup O107/O117 Lipopolysaccharide Binds and Neutralizes Shiga Toxin 2

Shantini D. Gamage, Colleen M. McGannon, and Alison A. Weiss*

Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, Cincinnati, Ohio 45267-0524

Received 12 March 2004/ Accepted 17 May 2004

The AB5 toxin Shiga toxin 2 (Stx2) has been implicated as a major virulence factor of Escherichia coli O157:H7 and other Shiga toxin-producing E. coli strains in the progression of intestinal disease to more severe systemic complications. Here, we demonstrate that supernatant from a normal E. coli isolate, FI-29, neutralizes the effect of Stx2, but not the related Stx1, on Vero cells. Biochemical characterization of the neutralizing activity identified the lipopolysaccharide (LPS) of FI-29, a serogroup O107/O117 strain, as the toxin-neutralizing component. LPSs from FI-29 as well as from type strains E. coli O107 and E. coli O117 were able bind Stx2 but not Stx1, indicating that the mechanism of toxin neutralization may involve inhibition of the interaction between Stx2 and the Gb3 receptor on Vero cells.

* Corresponding author. Mailing address: Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, OH 45267-0524. Phone: (513) 558-2820. Fax: (513) 558-8474. E-mail: Alison.Weiss{at}uc.edu.

Journal of Bacteriology, August 2004, p. 5506-5512, Vol. 186, No. 16
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.16.5506-5512.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Flagler, M. J., Strasser, J. E., Chalk, C. L., Weiss, A. A. (2007). Comparative Analysis of the Abilities of Shiga Toxins 1 and 2 To Bind to and Influence Neutrophil Apoptosis. Infect. Immun. 75: 760-765 [Abstract] [Full Text]  
  • Gamage, S. D., Patton, A. K., Strasser, J. E., Chalk, C. L., Weiss, A. A. (2006). Commensal Bacteria Influence Escherichia coli O157:H7 Persistence and Shiga Toxin Production in the Mouse Intestine. Infect. Immun. 74: 1977-1983 [Abstract] [Full Text]  


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