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Journal of Bacteriology, August 2004, p. 5523-5528, Vol. 186, No. 16
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.16.5523-5528.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Complete Genomic Nucleotide Sequence of the Temperate Bacteriophage Aa{Phi}23 of Actinobacillus actinomycetemcomitans{dagger}

Grégory Resch,1,{ddagger} Eva M. Kulik,1 Fred S. Dietrich,2,§ and Jürg Meyer1*

Institute for Preventive Dentistry and Oral Microbiology,1 Department of Applied Microbiology, Pharmacenter, University of Basel, 4056 Basel, Switzerland2

Received 13 June 2003/ Accepted 11 May 2004

The entire double-stranded DNA genome of the Actinobacillus actinomycetemcomitans bacteriophage Aa{Phi}23 was sequenced. Linear DNA contained in the phage particles is circularly permuted and terminally redundant. Therefore, the physical map of the phage genome is circular. Its size is 43,033 bp with an overall molar G+C content of 42.5 mol%. Sixty-six potential open reading frames (ORFs) were identified, including an ORF resulting from a translational frameshift. A putative function could be assigned to 23 of them. Twenty-three other ORFs share homologies only with hypothetical proteins present in several bacteria or bacteriophages, and 20 ORFs seem to be specific for phage Aa{Phi}23. The organization of the phage genome and several genetic functions share extensive similarities to that of the lambdoid phages. However, Aa{Phi}23 encodes a DNA adenine methylase, and the DNA packaging strategy is more closely related to the P22 system. The attachment sites of Aa{Phi}23 (attP) and several A. actinomycetemcomitans hosts (attB) are 49 bp long.

* Corresponding author. Mailing address: Institute for Preventive Dentistry and Oral Microbiology, Hebelstrasse 3, 4056 Basel, Switzerland. Phone: 41 61 2672601. Fax: 41 61 2672658. E-mail: juerg.meyer{at}unibas.ch.

{dagger} This paper is dedicated to Werner Arber on the occasion of his 75th birthday.

{ddagger} Present address: Biocenter, Department of Molecular Microbiology, 4056 Basel, Switzerland.

§ Present address: Center for Genome Technology, Duke University Medical Center, Durham, NC 27710.

Journal of Bacteriology, August 2004, p. 5523-5528, Vol. 186, No. 16
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.16.5523-5528.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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