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Journal of Bacteriology, September 2004, p. 5596-5602, Vol. 186, No. 17
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.17.5596-5602.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Functional Characterization of an Aminotransferase Required for Pyoverdine Siderophore Biosynthesis in Pseudomonas aeruginosa PAO1

Chris S. Vandenende, Matthew Vlasschaert, and Stephen Y. K. Seah*

Department of Microbiology, University of Guelph, Guelph, Ontario, Canada

Received 1 April 2004/ Accepted 30 May 2004

The fluorescent dihydroxyquinoline chromophore of the pyoverdine siderophore in Pseudomonas is a condensation product of D-tyrosine and L-2,4-diaminobutyrate. Both pvdH and asd (encoding aspartate ß-semialdehyde dehydrogenase) knockout mutants of Pseudomonas aeruginosa PAO1 were unable to synthesize pyoverdine under iron-limiting conditions in the absence of L-2,4-diaminobutyrate in the culture media. The pvdH gene was subcloned, and the gene product was hyperexpressed and purified from P. aeruginosa PAO1. PvdH was found to catalyze an aminotransferase reaction, interconverting aspartate ß-semialdehyde and L-2,4-diaminobutyrate. Steady-state kinetic analysis with a novel coupled assay established that the enzyme adopts a ping-pong kinetic mechanism and has the highest specificity for {alpha}-ketoglutarate. The specificity of the enzyme toward the smaller keto acid pyruvate is 41-fold lower. The enzyme has negligible activity toward other keto acids tested. Homologues of PvdH were present in the genomes of other Pseudomonas spp. These homologues were found in the DNA loci of the corresponding genomes that contain other pyoverdine synthesis genes. This suggests that there is a general mechanism of L-2,4-diaminobutyrate synthesis in Pseudomonas strains that produce the pyoverdine siderophore.

* Corresponding author. Mailing address: Department of Microbiology, University of Guelph, Guelph, Ontario, Canada N1G 2W1. Phone: (519) 824-4120, ext. 56750. Fax: (519) 837-1802. E-mail: sseah{at}uoguelph.ca.

Journal of Bacteriology, September 2004, p. 5596-5602, Vol. 186, No. 17
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.17.5596-5602.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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