This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yoshida, K.-i. Articles by Fujita, Y. Search for Related Content PubMed PubMed Citation Articles by Yoshida, K.-i. Articles by Fujita, Y.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 2004, p. 5640-5648, Vol. 186, No. 17
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.17.5640-5648.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Bacillus subtilis LmrA Is a Repressor of the lmrAB and yxaGH Operons: Identification of Its Binding Site and Functional Analysis of lmrB and yxaGH

Department of Biotechnology, Fukuyama University, Fukuyama, Hiroshima,¹ Department of Molecular Biology, School of Health Sciences, Kyorin University, Hachioji, Tokyo,² Institute of Biological Science, University of Tsukuba, Tsukuba, Ibaragi, Japan³

Received 15 January 2004/ Accepted 2 June 2004

The Bacillus subtilis lmrAB operon is involved in multidrug resistance. LmrA is a repressor of its own operon, while LmrB acts as a multidrug efflux transporter. LmrA was produced in Escherichia coli cells and was shown to bind to the lmr promoter region, in which an LmrA-binding site was identified. Genome-wide screening involving DNA microarray analysis allowed us to conclude that LmrA also repressed yxaGH, which was not likely to contribute to the multidrug resistance. LmrA bound to a putative yxaGH promoter region, in which two tandem LmrA-binding sites were identified. The LmrA regulon was thus determined to comprise lmrAB and yxaGH. All three LmrA-binding sites contained an 18-bp consensus sequence, TAGACCRKTCWMTATAWT, which could play an important role in LmrA binding.

This article has been cited by other articles:

• Hirooka, K., Kunikane, S., Matsuoka, H., Yoshida, K.-I., Kumamoto, K., Tojo, S., Fujita, Y. (2007). Dual Regulation of the Bacillus subtilis Regulon Comprising the lmrAB and yxaGH Operons and yxaF Gene by Two Transcriptional Repressors, LmrA and YxaF, in Response to Flavonoids. J. Bacteriol. 189: 5170-5182 [Abstract] [Full Text]  
• Le Breton, Y., Mohapatra, N. P., Haldenwang, W. G. (2006). In Vivo Random Mutagenesis of Bacillus subtilis by Use of TnYLB-1, a mariner-Based Transposon. Appl. Environ. Microbiol. 72: 327-333 [Abstract] [Full Text]  
• Guazzaroni, M.-E., Krell, T., Felipe, A., Ruiz, R., Meng, C., Zhang, X., Gallegos, M.-T., Ramos, J. L. (2005). The Multidrug Efflux Regulator TtgV Recognizes a Wide Range of Structurally Different Effectors in Solution and Complexed with Target DNA: EVIDENCE FROM ISOTHERMAL TITRATION CALORIMETRY. J. Biol. Chem. 280: 20887-20893 [Abstract] [Full Text]  
• Lin, J. T., Connelly, M. B., Amolo, C., Otani, S., Yaver, D. S. (2005). Global Transcriptional Response of Bacillus subtilis to Treatment with Subinhibitory Concentrations of Antibiotics That Inhibit Protein Synthesis. Antimicrob. Agents Chemother. 49: 1915-1926 [Abstract] [Full Text]  
• Ohki, R., Tateno, K. (2004). Increased Stability of bmr3 mRNA Results in a Multidrug-Resistant Phenotype in Bacillus subtilis. J. Bacteriol. 186: 7450-7455 [Abstract] [Full Text]