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Journal of Bacteriology, September 2004, p. 5856-5864, Vol. 186, No. 17
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.17.5856-5864.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The ClpP Peptidase Is the Major Determinant of Bulk Protein Turnover in Bacillus subtilis

Holger Kock, Ulf Gerth, and Michael Hecker*

Ernst-Moritz-Arndt-Universität Greifswald, Institut für Mikrobiologie, Greifswald, Germany

Received 11 March 2004/ Accepted 27 May 2004

Measurements of overall protein degradation rates in wild-type and clpP mutant Bacillus subtilis cells revealed that stress- or starvation-induced bulk protein turnover depends virtually exclusively on the ClpP peptidase. ClpP is also essential for intracellular protein quality control, and in its absence newly synthesized proteins were highly prone to aggregation even at 37°C. Proteomic comparisons between the wild type and a {Delta}clpP mutant showed that the absence of ClpP leads to severe perturbations of "normal" physiology, complicating the detection of ClpP substrates. A pulse-chase two-dimensional gel approach was therefore used to compare wild-type and clpP mutant cultures that had been radiolabeled in mid-exponential phase, by quantifying changes in relative spot intensities with time. The results showed that overall proteolysis is biased toward proteins with vegetative functions which are no longer required (or are required at lower levels) in the nongrowing state. The identified substrate candidates for ClpP-dependent degradation include metabolic enzymes and aminoacyl-tRNA synthetases. Some substrate candidates catalyze the first committed step of certain biosynthetic pathways. Our data suggest that ClpP-dependent proteolysis spans a broad physiological spectrum, with regulatory processing of key metabolic components and regulatory proteins on the one side and general bulk protein breakdown at the transition from growing to nongrowing phases on the other.

* Corresponding author. Mailing address: Ernst-Moritz-Arndt-Universität, Institut für Mikrobiologie, F.-L.-Jahn-Str. 15, 17487 Greifswald, Germany. Phone: 49-3834-864200. Fax: 49-3834-864202. E-mail: hecker{at}uni-greifswald.de.

Journal of Bacteriology, September 2004, p. 5856-5864, Vol. 186, No. 17
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.17.5856-5864.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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