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Characterization of the Upper Pathway Genes for Fluorene Metabolism in Terrabacter sp. Strain DBF63

Hiroshi Habe,^1* Jin-Sung Chung,¹ Hiroyuki Kato,¹ Yuko Ayabe,¹ Kano Kasuga,² Takako Yoshida,¹ Hideaki Nojiri,¹ Hisakazu Yamane,¹ and Toshio Omori^1,

Biotechnology Research Center, The University of Tokyo, Bunkyo-ku, Tokyo,¹ Department of Biotechnology, Faculty of Bioresource Sciences, Akita Prefectural University, Shimoshinjo-nakano, Akita, Japan²

Received 27 May 2004/ Accepted 8 June 2004

Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activities against both 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone to produce 9-fluorenone and 2'-carboxy-2,3-dihydroxybiphenyl, respectively. FlnD is a heteromeric protein encoded by flnD1 and ORF16, being a member of the class III two-subunit extradiol dioxygenase. FlnE was identified as a serine hydrolase for the meta-cleavage products that yield phthalate.

Present address: Department of Industrial Chemistry, Faculty of Engineering, Shibaura Institute of Technology, Minato-ku, Tokyo 108-8548, Japan.

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