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Journal of Bacteriology, September 2004, p. 5961-5967, Vol. 186, No. 17
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.17.5961-5967.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Mutational Analysis of the fruA Promoter Region Demonstrates that C-Box and 5-Base-Pair Elements Are Important for Expression of an Essential Developmental Gene of Myxococcus xanthus

D. Srinivasan and Lee Kroos*

Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan

Received 15 March 2004/ Accepted 26 May 2004

Myxococcus xanthus uses extracellular signals during development to regulate gene expression. C-signaling regulates the expression of many genes induced after 6 h into development. FruA is a protein that is necessary for cells to respond to C-signaling, but expression of the fruA gene does not depend on C-signaling. Yet the fruA promoter region has a C box and a 5-bp element, similar to the promoter regions of several C-signal-dependent genes, where these sequences are crucial. Here, we show that the C box and 5-bp elements are important for expression of fruA, demonstrating for the first time that these sequences play a role in the expression of a gene that does not depend on C-signaling and is required for M. xanthus development.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824. Phone: (517) 355-9726. Fax: (517) 353-9334. E-mail: kroos{at}pilot.msu.edu.

Journal of Bacteriology, September 2004, p. 5961-5967, Vol. 186, No. 17
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.17.5961-5967.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mittal, S., Kroos, L. (2009). Combinatorial Regulation by a Novel Arrangement of FruA and MrpC2 Transcription Factors during Myxococcus xanthus Development. J. Bacteriol. 191: 2753-2763 [Abstract] [Full Text]  
  • Viswanathan, P., Murphy, K., Julien, B., Garza, A. G., Kroos, L. (2007). Regulation of dev, an Operon That Includes Genes Essential for Myxococcus xanthus Development and CRISPR-Associated Genes and Repeats. J. Bacteriol. 189: 3738-3750 [Abstract] [Full Text]  
  • Gronewold, T. M. A., Kaiser, D. (2007). Mutations of the Act Promoter in Myxococcus xanthus. J. Bacteriol. 189: 1836-1844 [Abstract] [Full Text]  
  • Viswanathan, P., Singer, M., Kroos, L. (2006). Role of {sigma}D in Regulating Genes and Signals during Myxococcus xanthus Development. J. Bacteriol. 188: 3246-3256 [Abstract] [Full Text]  
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