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Journal of Bacteriology, September 2004, p. 6306-6310, Vol. 186, No. 18
0021-9193/04/$08.00+0 DOI: 10.1128/JB.186.18.6306-6310.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
Department of Microbiology, Ohio State University, Columbus, Ohio
Received 18 May 2004/ Accepted 28 June 2004
Transcription initiation in Archaea requires the assembly of a preinitiation complex containing the TATA- box binding protein (TBP), transcription factor B (TFB), and RNA polymerase (RNAP). The results reported establish the fate of Methanothermobacter thermautotrophicus TBP and TFB following transcription initiation by M. thermautotrophicus RNAP in vitro. TFB is released after initiation, during extension of the transcript from 4 to 24 nucleotides, but TBP remains bound to the template DNA. Regulation of archaeal transcription initiation by a repressor competition with TBP for TATA-box region binding must accommodate this observation.
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