This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Xie, Y. Articles by Reeve, J. N. Search for Related Content PubMed PubMed Citation Articles by Xie, Y. Articles by Reeve, J. N.

Transcription by Methanothermobacter thermautotrophicus RNA Polymerase In Vitro Releases Archaeal Transcription Factor B but Not TATA-Box Binding Protein from the Template DNA

Department of Microbiology, Ohio State University, Columbus, Ohio

Received 18 May 2004/ Accepted 28 June 2004

Transcription initiation in Archaea requires the assembly of a preinitiation complex containing the TATA- box binding protein (TBP), transcription factor B (TFB), and RNA polymerase (RNAP). The results reported establish the fate of Methanothermobacter thermautotrophicus TBP and TFB following transcription initiation by M. thermautotrophicus RNAP in vitro. TFB is released after initiation, during extension of the transcript from 4 to 24 nucleotides, but TBP remains bound to the template DNA. Regulation of archaeal transcription initiation by a repressor competition with TBP for TATA-box region binding must accommodate this observation.

This article has been cited by other articles:

• Karr, E. A., Sandman, K., Lurz, R., Reeve, J. N. (2008). TrpY Regulation of trpB2 Transcription in Methanothermobacter thermautotrophicus. J. Bacteriol. 190: 2637-2641 [Abstract] [Full Text]  
• Xie, Y., Reeve, J. N. (2005). Regulation of Tryptophan Operon Expression in the Archaeon Methanothermobacter thermautotrophicus. J. Bacteriol. 187: 6419-6429 [Abstract] [Full Text]