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Journal of Bacteriology, September 2004, p. 6311-6315, Vol. 186, No. 18
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.18.6311-6315.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

mRNA Secondary Structure Modulates Translation of Tat-Dependent Formate Dehydrogenase N

Claire Punginelli,1 Bérengère Ize,1,2 Nicola R. Stanley,1,2,{dagger} Valley Stewart,3 Gary Sawers,1 Ben C. Berks,4 and Tracy Palmer1,2*

Department of Molecular Microbiology, John Innes Centre,1 School of Biological Sciences, University of East Anglia, Norwich,2 Department of Biochemistry, University of Oxford, Oxford, United Kingdom,4 Section of Microbiology, University of California, Davis, California3

Received 14 May 2004/ Accepted 14 June 2004

Formate dehydrogenase N (FDH-N) of Escherichia coli is a membrane-bound enzyme comprising FdnG, FdnH, and FdnI subunits organized in an ({alpha}ß{gamma})3 configuration. The FdnG subunit carries a Tat-dependent signal peptide, which localizes the protein complex to the periplasmic side of the membrane. We noted that substitution of the first arginine (R5) in the twin arginine signal sequence of FdnG for a variety of other amino acids resulted in a dramatic (up to 60-fold) increase in the levels of protein synthesized. Bioinformatic analysis suggested that the mRNA specifying the first 17 codons of fdnG forms a stable stem-loop structure. A detailed mutational analysis has demonstrated the importance of this mRNA stem-loop in modulating FDH-N translation.

* Corresponding author. Mailing address: Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich NR4 7UH, United Kingdom. Fax: (44) (1603) 450778. E-mail: tracy.palmer{at}bbsrc.ac.uk.

{dagger} Present address: Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095-1489.

Journal of Bacteriology, September 2004, p. 6311-6315, Vol. 186, No. 18
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.18.6311-6315.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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  • Kim, J.-Y., Fogarty, E. A., Lu, F. J., Zhu, H., Wheelock, G. D., Henderson, L. A., DeLisa, M. P. (2005). Twin-Arginine Translocation of Active Human Tissue Plasminogen Activator in Escherichia coli. Appl. Environ. Microbiol. 71: 8451-8459 [Abstract] [Full Text]  


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