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Journal of Bacteriology, September 2004, p. 6320-6324, Vol. 186, No. 18
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.18.6320-6324.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Secretion Signal of YopN, a Regulatory Protein of the Yersinia enterocolitica Type III Secretion Pathway{dagger}

John W. Goss, Joseph A. Sorg, Kumaran S. Ramamurthi, Hung Ton-That, and Olaf Schneewind*

Committee on Microbiology, University of Chicago, Chicago, Illinois

Received 2 April 2004/ Accepted 7 June 2004

The type III secretion signal of Yersinia enterocolitica YopN was mapped using a gene fusion approach. yopN codons 1 to 12 were identified as critical for signal function. Several synonymous mutations that abolish secretion of hybrid proteins without altering the codon specificity of yopN mRNA were identified.


* Corresponding author. Mailing address: Committee on Microbiology, The University of Chicago, 920 E. 58th St., Chicago, IL 60637. Phone: (773) 834-9060. Fax: (773) 834-8150. E-mail: oschnee{at}bsd.uchicago.edu.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.


Journal of Bacteriology, September 2004, p. 6320-6324, Vol. 186, No. 18
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.18.6320-6324.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.




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