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Journal of Bacteriology, October 2004, p. 6526-6535, Vol. 186, No. 19
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.19.6526-6535.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Activation of the Rcs Signal Transduction System Is Responsible for the Thermosensitive Growth Defect of an Escherichia coli Mutant Lacking Phosphatidylglycerol and Cardiolipin

Yasuhiro Shiba, Yasuko Yokoyama, Yoshiko Aono, Takashi Kiuchi,{dagger} Jin Kusaka, Kouji Matsumoto, and Hiroshi Hara*

Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, Saitama, Japan

Received 15 May 2004/ Accepted 8 July 2004

The lethal effect of an Escherichia coli pgsA null mutation, which causes a complete lack of the major acidic phospholipids, phosphatidylglycerol and cardiolipin, is alleviated by a lack of the major outer membrane lipoprotein encoded by the lpp gene, but an lpp pgsA strain shows a thermosensitive growth defect. Using transposon mutagenesis, we found that this thermosensitivity was suppressed by disruption of the rcsC, rcsF, and yojN genes, which code for a sensor kinase, accessory positive factor, and phosphotransmitter, respectively, of the Rcs phosphorelay signal transduction system initially identified as regulating the capsular polysaccharide synthesis (cps) genes. Disruption of the rcsB gene coding for the response regulator of the system also suppressed the thermosensitivity, whereas disruption of cpsE did not. By monitoring the expression of a cpsB'-lac fusion, we showed that the Rcs system is activated in the pgsA mutant and is reverted to a wild-type level by the rcs mutations. These results indicate that envelope stress due to an acidic phospholipid deficiency activates the Rcs phosphorelay system and thereby causes the thermosensitive growth defect independent of the activation of capsule synthesis.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, 255 Shimo-ohkubo, Sakura-ku, Saitama-shi, Saitama-ken 338-8570, Japan. Phone: 81-48-858-3775. Fax: 81-48-858-3384. E-mail: hhara{at}post.saitama-u.ac.jp.

{dagger} Present address: Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Tokyo, Japan.

Journal of Bacteriology, October 2004, p. 6526-6535, Vol. 186, No. 19
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.19.6526-6535.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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