Previous Article | Next Article 
Journal of Bacteriology, October 2004, p. 6656-6660, Vol. 186, No. 19
0021-9193/04/$08.00+0 DOI: 10.1128/JB.186.19.6656-6660.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
DNA Binding Activity of the Escherichia coli Nitric Oxide Sensor NorR Suggests a Conserved Target Sequence in Diverse Proteobacteria
Nicholas P. Tucker,1 Benoît D'Autréaux,1 David J. Studholme,2 Stephen Spiro,3* and Ray Dixon1
John Innes Centre, Colney, Norwich,1
Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom,2
School of Biology, Georgia Institute of Technology, Atlanta, Georgia3
Received 6 May 2004/
Accepted 7 July 2004
The Escherichia coli nitric oxide sensor NorR was shown to bind to the promoter region of the norVW transcription unit, forming at least two distinct complexes detectable by gel retardation. Three binding sites for NorR and two integration host factor binding sites were identified in the norR-norV intergenic region. The derived consensus sequence for NorR binding sites was used to search for novel members of the E. coli NorR regulon and to show that NorR binding sites are partially conserved in other members of the proteobacteria.
* Corresponding author. Mailing address: School of Biology, Georgia Institute of Technology, 310 Ferst Dr., Atlanta, GA 30332-0230. Phone: (404) 385-6313. Fax: (404) 894-0519. E-mail:
stephen.spiro{at}biology.gatech.edu.
Journal of Bacteriology, October 2004, p. 6656-6660, Vol. 186, No. 19
0021-9193/04/$08.00+0 DOI: 10.1128/JB.186.19.6656-6660.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Tucker, N. P., D'Autreaux, B., Yousafzai, F. K., Fairhurst, S. A., Spiro, S., Dixon, R.
(2008). Analysis of the Nitric Oxide-sensing Non-heme Iron Center in the NorR Regulatory Protein. J. Biol. Chem.
283: 908-918
[Abstract]
[Full Text]
-
Rodrigues, R., Vicente, J. B., Felix, R., Oliveira, S., Teixeira, M., Rodrigues-Pousada, C.
(2006). Desulfovibrio gigas Flavodiiron Protein Affords Protection against Nitrosative Stress In Vivo.. J. Bacteriol.
188: 2745-2751
[Abstract]
[Full Text]
-
Bodenmiller, D. M., Spiro, S.
(2006). The yjeB (nsrR) Gene of Escherichia coli Encodes a Nitric Oxide-Sensitive Transcriptional Regulator. J. Bacteriol.
188: 874-881
[Abstract]
[Full Text]
-
Diaz-Mireles, E., Wexler, M., Todd, J. D., Bellini, D., Johnston, A. W. B., Sawers, R. G.
(2005). The manganese-responsive repressor Mur of Rhizobium leguminosarum is a member of the Fur-superfamily that recognizes an unusual operator sequence. Microbiology
151: 4071-4078
[Abstract]
[Full Text]
-
Gabrovsky, V., Yamamoto, M. L., Miller, J. H.
(2005). Mutator Effects in Escherichia coli Caused by the Expression of Specific Foreign Genes. J. Bacteriol.
187: 5044-5048
[Abstract]
[Full Text]
-
Arai, H., Hayashi, M., Kuroi, A., Ishii, M., Igarashi, Y.
(2005). Transcriptional Regulation of the Flavohemoglobin Gene for Aerobic Nitric Oxide Detoxification by the Second Nitric Oxide-Responsive Regulator of Pseudomonas aeruginosa. J. Bacteriol.
187: 3960-3968
[Abstract]
[Full Text]
-
Justino, M. C., Vicente, J. B., Teixeira, M., Saraiva, L. M.
(2005). New Genes Implicated in the Protection of Anaerobically Grown Escherichia coli against Nitric Oxide. J. Biol. Chem.
280: 2636-2643
[Abstract]
[Full Text]