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Journal of Bacteriology, January 2004, p. 287-295, Vol. 186, No. 2
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.2.287-295.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Methyltransferase from the LlaDII Restriction-Modification System Influences the Level of Expression of Its Own Gene

Lisa Lystbæk Christensen and Jytte Josephsen*

Department of Dairy and Food Science, Centre of Advanced Food Studies, The Royal Veterinary and Agricultural University, DK-1958 Frederiksberg C, Denmark

Received 14 July 2003/ Accepted 13 October 2003

The type II restriction-modification (R-M) system LlaDII isolated from Lactococcus lactis contains two tandemly arranged genes, llaDIIR and llaDIIM, encoding a restriction endonuclease (REase) and a methyltransferase (MTase), respectively. Interestingly, two LlaDII recognition sites are present in the llaDIIM promoter region, suggesting that they may influence the activity of the promoter through methylation status. In this study, separate promoters for llaDIIR and llaDIIM were identified, and the regulation of the two genes at the transcriptional level was investigated. DNA fragments containing the putative promoters were cloned in a promoter probe vector and tested for activity in the presence and absence of the active MTase. The level of expression of the MTase was 5- to 10-fold higher than the level of expression of the REase. The results also showed that the presence of M.LlaDII reduced the in vivo expression of the llaDIIM promoter (PllaDIIM) up to 1,000-fold, whereas the activity of the llaDIIR promoter (PllaDIIR) was not affected. Based on site-specific mutations it was shown that both of the LlaDII recognition sites within PllaDIIM are required to obtain complete repression of transcriptional activity. No regulation was found for llaDIIR, which appears to be constitutively expressed.

* Corresponding author. Mailing address: Department of Dairy and Food Science, The Royal Veterinary and Agricultural University, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark. Phone: 45 35 28 32 32. Fax: 45 35 28 32 14. E-mail: jyj{at}kvl.dk.

Journal of Bacteriology, January 2004, p. 287-295, Vol. 186, No. 2
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.2.287-295.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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