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Journal of Bacteriology, January 2004, p. 570-574, Vol. 186, No. 2
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.2.570-574.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Cloning and Characterization of Acetohydroxyacid Synthase from Bacillus stearothermophilus

Iris Porat,1,{dagger} Michael Vinogradov,1 Maria Vyazmensky,1 Chung-Dar Lu,2 David M. Chipman,1 Ahmed T. Abdelal,3 and Ze'ev Barak1*

Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, 84105 Israel,1 Biology Department, Northeastern University, Boston, Massachusetts 02115-5000,3 Department of Biology, Georgia State University, Atlanta, Georgia 303032

Received 20 June 2003/ Accepted 3 October 2003

Five genes from the ilv-leu operon from Bacillus stearothermophilus have been sequenced. Acetohydroxyacid synthase (AHAS) and its subunits were separately cloned, purified, and characterized. This thermophilic enzyme resembles AHAS III of Escherichia coli, and regulatory subunits of AHAS III complement the catalytic subunit of the AHAS of B. stearothermophilus, suggesting that AHAS III is functionally and evolutionally related to the single AHAS of gram-positive bacteria.

* Corresponding author. Mailing address: Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva, 84105 Israel. Phone: (972) 864 61713. Fax: (972) 864 79178. E-mail: barakz{at}bgumail.bgu.ac.il.

{dagger} Present address: Department of Microbiology, University of Georgia, Athens, GA 30602-2605.

Journal of Bacteriology, January 2004, p. 570-574, Vol. 186, No. 2
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.2.570-574.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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