This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Henke, J. M.
Right arrow Articles by Bassler, B. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Henke, J. M.
Right arrow Articles by Bassler, B. L.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2004, p. 6902-6914, Vol. 186, No. 20
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.20.6902-6914.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Three Parallel Quorum-Sensing Systems Regulate Gene Expression in Vibrio harveyi{dagger}

Jennifer M. Henke and Bonnie L. Bassler*

Department of Molecular Biology, Princeton University, Princeton, New Jersey

Received 3 June 2004/ Accepted 12 July 2004

In a process called quorum sensing, bacteria communicate using extracellular signal molecules termed autoinducers. Two parallel quorum-sensing systems have been identified in the marine bacterium Vibrio harveyi. System 1 consists of the LuxM-dependent autoinducer HAI-1 and the HAI-1 sensor, LuxN. System 2 consists of the LuxS-dependent autoinducer AI-2 and the AI-2 detector, LuxPQ. The related bacterium, Vibrio cholerae, a human pathogen, possesses System 2 (LuxS, AI-2, and LuxPQ) but does not have obvious homologues of V. harveyi System 1. Rather, System 1 of V. cholerae is made up of the CqsA-dependent autoinducer CAI-1 and a sensor called CqsS. Using a V. cholerae CAI-1 reporter strain we show that many other marine bacteria, including V. harveyi, produce CAI-1 activity. Genetic analysis of V. harveyi reveals cqsA and cqsS, and phenotypic analysis of V. harveyi cqsA and cqsS mutants shows that these functions comprise a third V. harveyi quorum-sensing system that acts in parallel to Systems 1 and 2. Together these communication systems act as a three-way coincidence detector in the regulation of a variety of genes, including those responsible for bioluminescence, type III secretion, and metalloprotease production.

* Corresponding author. Mailing address: Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014. Phone: (609) 258-2857. Fax: (609) 258-6175. E-mail: bbassler{at}molbio.princeton.edu.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, October 2004, p. 6902-6914, Vol. 186, No. 20
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.20.6902-6914.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Atkinson, S., Williams, P. (2009). Quorum sensing and social networking in the microbial world. J R Soc Interface 6: 959-978 [Abstract] [Full Text]  
  • Wegener-Feldbrugge, S., Sogaard-Andersen, L. (2009). The Atypical Hybrid Histidine Protein Kinase RodK in Myxococcus xanthus: Spatial Proximity Supersedes Kinetic Preference in Phosphotransfer Reactions. J. Bacteriol. 191: 1765-1776 [Abstract] [Full Text]  
  • Teasdale, M. E., Liu, J., Wallace, J., Akhlaghi, F., Rowley, D. C. (2009). Secondary Metabolites Produced by the Marine Bacterium Halobacillus salinus That Inhibit Quorum Sensing-Controlled Phenotypes in Gram-Negative Bacteria. Appl. Environ. Microbiol. 75: 567-572 [Abstract] [Full Text]  
  • Hammer, B. K., Bassler, B. L. (2009). Distinct Sensory Pathways in Vibrio cholerae El Tor and Classical Biotypes Modulate Cyclic Dimeric GMP Levels To Control Biofilm Formation. J. Bacteriol. 191: 169-177 [Abstract] [Full Text]  
  • Lebeer, S., Vanderleyden, J., De Keersmaecker, S. C. J. (2008). Genes and Molecules of Lactobacilli Supporting Probiotic Action. Microbiol. Mol. Biol. Rev. 72: 728-764 [Abstract] [Full Text]  
  • Tiaden, A., Spirig, T., Carranza, P., Bruggemann, H., Riedel, K., Eberl, L., Buchrieser, C., Hilbi, H. (2008). Synergistic Contribution of the Legionella pneumophila lqs Genes to Pathogen-Host Interactions. J. Bacteriol. 190: 7532-7547 [Abstract] [Full Text]  
  • Studer, S. V., Mandel, M. J., Ruby, E. G. (2008). AinS Quorum Sensing Regulates the Vibrio fischeri Acetate Switch. J. Bacteriol. 190: 5915-5923 [Abstract] [Full Text]  
  • Spirig, T., Tiaden, A., Kiefer, P., Buchrieser, C., Vorholt, J. A., Hilbi, H. (2008). The Legionella Autoinducer Synthase LqsA Produces an {alpha}-Hydroxyketone Signaling Molecule. J. Biol. Chem. 283: 18113-18123 [Abstract] [Full Text]  
  • Pottathil, M., Jung, A., Lazazzera, B. A. (2008). CSF, a Species-Specific Extracellular Signaling Peptide for Communication among Strains of Bacillus subtilis and Bacillus mojavensis. J. Bacteriol. 190: 4095-4099 [Abstract] [Full Text]  
  • Weber, B., Croxatto, A., Chen, C., Milton, D. L. (2008). RpoS induces expression of the Vibrio anguillarum quorum-sensing regulator VanT. Microbiology 154: 767-780 [Abstract] [Full Text]  
  • Liang, W., Pascual-Montano, A., Silva, A. J., Benitez, J. A. (2007). The cyclic AMP receptor protein modulates quorum sensing, motility and multiple genes that affect intestinal colonization in Vibrio cholerae. Microbiology 153: 2964-2975 [Abstract] [Full Text]  
  • Williams, P., Winzer, K., Chan, W. C, Camara, M. (2007). Look who's talking: communication and quorum sensing in the bacterial world. Phil Trans R Soc B 362: 1119-1134 [Abstract] [Full Text]  
  • Barnard, A. M.L, Bowden, S. D, Burr, T., Coulthurst, S. J, Monson, R. E, Salmond, G. P.C (2007). Quorum sensing, virulence and secondary metabolite production in plant soft-rotting bacteria. Phil Trans R Soc B 362: 1165-1183 [Abstract] [Full Text]  
  • Hammer, B. K., Bassler, B. L. (2007). Inaugural Article: Regulatory small RNAs circumvent the conventional quorum sensing pathway in pandemic Vibrio cholerae. Proc. Natl. Acad. Sci. USA 104: 11145-11149 [Abstract] [Full Text]  
  • Shemesh, M., Tam, A., Steinberg, D. (2007). Differential gene expression profiling of Streptococcus mutans cultured under biofilm and planktonic conditions. Microbiology 153: 1307-1317 [Abstract] [Full Text]  
  • Coenye, T., Honraet, K., Rigole, P., Jimenez, P. N., Nelis, H. J. (2007). In Vitro Inhibition of Streptococcus mutans Biofilm Formation on Hydroxyapatite by Subinhibitory Concentrations of Anthraquinones. Antimicrob. Agents Chemother. 51: 1541-1544 [Abstract] [Full Text]  
  • Jung, K., Odenbach, T., Timmen, M. (2007). The Quorum-Sensing Hybrid Histidine Kinase LuxN of Vibrio harveyi Contains a Periplasmically Located N Terminus. J. Bacteriol. 189: 2945-2948 [Abstract] [Full Text]  
  • Tu, K. C., Bassler, B. L. (2007). Multiple small RNAs act additively to integrate sensory information and control quorum sensing in Vibrio harveyi. Genes Dev. 21: 221-233 [Abstract] [Full Text]  
  • Gonzalez, J. E., Keshavan, N. D. (2006). Messing with Bacterial Quorum Sensing. Microbiol. Mol. Biol. Rev. 70: 859-875 [Abstract] [Full Text]  
  • Roh, J.-B., Lee, M.-A., Lee, H.-J., Kim, S.-M., Cho, Y., Kim, Y.-J., Seok, Y.-J., Park, S.-J., Lee, K.-H. (2006). Transcriptional Regulatory Cascade for Elastase Production in Vibrio vulnificus: LuxO ACTIVATES luxT EXPRESSION AND LuxT REPRESSES smcR EXPRESSION. J. Biol. Chem. 281: 34775-34784 [Abstract] [Full Text]  
  • Krin, E., Chakroun, N., Turlin, E., Givaudan, A., Gaboriau, F., Bonne, I., Rousselle, J.-C., Frangeul, L., Lacroix, C., Hullo, M.-F., Marisa, L., Danchin, A., Derzelle, S. (2006). Pleiotropic Role of Quorum-Sensing Autoinducer 2 in Photorhabdus luminescens.. Appl. Environ. Microbiol. 72: 6439-6451 [Abstract] [Full Text]  
  • Waters, C. M., Bassler, B. L. (2006). The Vibrio harveyi quorum-sensing system uses shared regulatory components to discriminate between multiple autoinducers. Genes Dev. 20: 2754-2767 [Abstract] [Full Text]  
  • Defoirdt, T., Crab, R., Wood, T. K., Sorgeloos, P., Verstraete, W., Bossier, P. (2006). Quorum Sensing-Disrupting Brominated Furanones Protect the Gnotobiotic Brine Shrimp Artemia franciscana from Pathogenic Vibrio harveyi, Vibrio campbellii, and Vibrio parahaemolyticus Isolates. Appl. Environ. Microbiol. 72: 6419-6423 [Abstract] [Full Text]  
  • Timmen, M., Bassler, B. L., Jung, K. (2006). AI-1 Influences the Kinase Activity but Not the Phosphatase Activity of LuxN of Vibrio harveyi. J. Biol. Chem. 281: 24398-24404 [Abstract] [Full Text]  
  • Auchtung, J. M., Lee, C. A., Grossman, A. D. (2006). Modulation of the ComA-Dependent Quorum Response in Bacillus subtilis by Multiple Rap Proteins and Phr Peptides.. J. Bacteriol. 188: 5273-5285 [Abstract] [Full Text]  
  • Jaques, S., McCarter, L. L. (2006). Three New Regulators of Swarming in Vibrio parahaemolyticus.. J. Bacteriol. 188: 2625-2635 [Abstract] [Full Text]  
  • Doherty, N., Holden, M. T. G., Qazi, S. N., Williams, P., Winzer, K. (2006). Functional Analysis of luxS in Staphylococcus aureus Reveals a Role in Metabolism but Not Quorum Sensing.. J. Bacteriol. 188: 2885-2897 [Abstract] [Full Text]  
  • Klose, K. E. (2006). Increased Chatter: Cyclic Dipeptides as Molecules of Chemical Communication in Vibrio spp.. J. Bacteriol. 188: 2025-2026 [Full Text]  
  • Romao, S., Memmi, G., Oggioni, M. R., Trombe, M.-C. (2006). LuxS impacts on LytA-dependent autolysis and on competence in Streptococcus pneumoniae. Microbiology 152: 333-341 [Abstract] [Full Text]  
  • Herzberg, M., Kaye, I. K., Peti, W., Wood, T. K. (2006). YdgG (TqsA) Controls Biofilm Formation in Escherichia coli K-12 through Autoinducer 2 Transport. J. Bacteriol. 188: 587-598 [Abstract] [Full Text]  
  • Xu, L., Li, H., Vuong, C., Vadyvaloo, V., Wang, J., Yao, Y., Otto, M., Gao, Q. (2006). Role of the luxS Quorum-Sensing System in Biofilm Formation and Virulence of Staphylococcus epidermidis. Infect. Immun. 74: 488-496 [Abstract] [Full Text]  
  • Visick, K. L., Fuqua, C. (2005). Decoding Microbial Chatter: Cell-Cell Communication in Bacteria. J. Bacteriol. 187: 5507-5519 [Full Text]  
  • Visick, K. L. (2005). Layers of Signaling in a Bacterium-Host Association. J. Bacteriol. 187: 3603-3606 [Full Text]  
  • Lin, W., Kovacikova, G., Skorupski, K. (2005). Requirements for Vibrio cholerae HapR Binding and Transcriptional Repression at the hapR Promoter Are Distinct from Those at the aphA Promoter. J. Bacteriol. 187: 3013-3019 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»