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Journal of Bacteriology, October 2004, p. 7015-7018, Vol. 186, No. 20
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.20.7015-7018.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evidence for Polyadenylated mRNA in Pseudomonas aeruginosa

Senthil Selvan Saravanamuthu,1 Franz von Götz,1,{dagger} Prabhakar Salunkhe,2,{dagger} Rathinam Chozhavendan,3 Robert Geffers,4 Jan Buer,1,4 Burkhard Tümmler,2 and Ivo Steinmetz1*

Institute for Medical Microbiology and Hospital Epidemiology,1 Clinical Research Group,2 Department for Pediatric Hematology and Oncology, Medical School Hannover, Hannover,3 German Research Centre for Biotechnology, Braunschweig, Germany4

Received 29 June 2004/ Accepted 5 July 2004

In this paper, we report the synthesis of Pseudomonas aeruginosa cDNA in the presence of oligo(dT) primers. Hybridization of oligonucleotide DNA microarrays indicates that under the experimental conditions used, at least 43.7% of the expressed genes from P. aeruginosa PAO1, representing many different functional classes, can be detected by using oligo(dT)-primed cDNAs.

* Corresponding author. Present address: Friedrich Loeffler Institute for Medical Microbiology, Ernst Moritz Arndt-University Greifswald, Martin-Luther-Straße 6, 17487 Griefswald, Germany. Phone: (49) 3834-865560. Fax: (49) 3834-865561. E-mail: steinmetz.ivo{at}uni-griefswald.de.

{dagger} F.V.G. and P.S. contributed equally to this work.

Journal of Bacteriology, October 2004, p. 7015-7018, Vol. 186, No. 20
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.20.7015-7018.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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