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Journal of Bacteriology, November 2004, p. 7460-7465, Vol. 186, No. 21
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.21.7460-7465.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Molecular Basis of the Indole-Negative Reaction in Shigella Strains: Extensive Damages to the tna Operon by Insertion Sequences

Ferdousi Rezwan,1 Ruiting Lan,2 and Peter R. Reeves1*

School of Molecular and Microbial Biosciences, University of Sydney,1 School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia2

Received 24 March 2004/ Accepted 5 August 2004

The molecular basis of the loss of tryptophan utilization (indole-negative phenotype) of Shigella strains, in effect clones of Escherichia coli, was investigated. Analysis of the tna operon of 23 Shigella strains representing each of the indole-negative serotypes revealed that insertion sequence-mediated insertion and/or deletions damaged the tna operon, leading to inability to convert tryptophan to indole. These events differ for cluster 1, cluster 3, and the outlier Shigella strains, confirming our previous observation of independent origins of these lineages from within E. coli. Parallel loss of the trait and prevalence of indole-negative strains suggest that the trait is deleterious in Shigella strains and advantages those without it.

* Corresponding author. Mailing address: School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia. Phone: 61 2 9351 6045. Fax: 61 2 9351 4571. E-mail: reeves{at}angis.usyd.edu.au.

Journal of Bacteriology, November 2004, p. 7460-7465, Vol. 186, No. 21
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.21.7460-7465.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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