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Journal of Bacteriology, November 2004, p. 7571-7574, Vol. 186, No. 22
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.22.7571-7574.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Chlamydiaphage Chp2, a Skeleton in the {phi}X174 Closet: Scaffolding Protein and Procapsid Identification

Ian N. Clarke,1 Leslie T. Cutcliffe,1 J. Sylvia Everson,1 Sarah A. Garner,1 Paul R. Lambden,1 Paddy J. Pead,1 Mark A. Pickett,1 Karie L. Brentlinger,2 and Bentley A. Fane2*

Molecular Microbiology Group, University Medical School, Southampton General Hospital, Southampton, United Kingdom,1 Department of Veterinary Sciences and Microbiology, University of Arizona, Tucson, Arizona2

Received 29 April 2004/ Accepted 11 August 2004

Chlamydiaphage Chp2 is a member of the family Microviridae, of which bacteriophage {phi}X174 is the type species. Although grouped in the same family, the relationship between the Microviridae coliphages and the Chp2-like viruses, which infect obligate intracellular parasitic bacteria, is quite distant, with major differences in structural protein content and scaffolding protein dependence. To investigate the morphogenesis of Chp2, large particles were isolated from infected Chlamydophila abortus by equilibrium and rate zonal sedimentation. A monoclonal antibody that recognizes only assembled viral coat proteins was used in these detection assays. Thus, the detected particles represent virions and/or postcapsid formation assembly intermediates. Two distinct particle types were detected, differing in both protein and DNA content. Filled particles lacked VP3, the putative internal scaffolding protein, whereas empty particles contained this protein. These results indicate that VP3 is a scaffolding protein and that the isolated VP3-containing particles most likely represent Chp2 procapsids.

* Corresponding author. Mailing address: Department of Veterinary Sciences and Microbiology, Building 90, University of Arizona, Tucson, AZ 85721-0090. Phone: (502) 626-6634. Fax: (520) 621-6636. E-mail: bfane{at}u.arizona.edu.

Journal of Bacteriology, November 2004, p. 7571-7574, Vol. 186, No. 22
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.22.7571-7574.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Skilton, R. J., Cutcliffe, L. T., Pickett, M. A., Lambden, P. R., Fane, B. A., Clarke, I. N. (2007). Intracellular Parasitism of Chlamydiae: Specific Infectivity of Chlamydiaphage Chp2 in Chlamydophila abortus. J. Bacteriol. 189: 4957-4959 [Abstract] [Full Text]  


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