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Journal of Bacteriology, November 2004, p. 7645-7652, Vol. 186, No. 22
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.22.7645-7652.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Molecular Identification of the Urea Uptake System and Transcriptional Analysis of Urea Transporter- and Urease-Encoding Genes in Corynebacterium glutamicum

Gabriele Beckers, Anne K. Bendt, Reinhard Krämer, and Andreas Burkovski^*

Institut für Biochemie der Universität zu Köln, Cologne, Germany

Received 9 July 2004/ Accepted 16 August 2004

The molecular identification of the Corynebacterium glutamicum urea uptake system is described. This ABC-type transporter is encoded by the urtABCDE operon, which is transcribed in response to nitrogen limitation. Expression of the urt genes is regulated by the global nitrogen regulator AmtR, and an amtR deletion strain showed constitutive expression of the urtABCDE genes. The AmtR repressor protein also controls transcription of the urease-encoding ureABCEFGD genes in C. glutamicum. The ure gene cluster forms an operon which is mainly transcribed in response to nitrogen starvation. To confirm the increased synthesis of urease subunits under nitrogen limitation, proteome analyses of cytoplasmic protein extracts from cells grown under nitrogen surplus and nitrogen limitation were carried out, and five of the seven urease subunits were identified.

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* Corresponding author. Mailing address: Institut für Biochemie der Universität zu Köln, Zülpicher-Str. 47, D-50674 Köln, Germany. Phone: 49 221 470 6472. Fax: 49 221 470 5091. E-mail: a.burkovski{at}uni-koeln.de.

Present address: Department of Biochemistry, National University of Singapore, Singapore 117597.

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Journal of Bacteriology, November 2004, p. 7645-7652, Vol. 186, No. 22
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.22.7645-7652.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

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