This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Salama, N. R.
Right arrow Articles by Falkow, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Salama, N. R.
Right arrow Articles by Falkow, S.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2004, p. 7926-7935, Vol. 186, No. 23
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.23.7926-7935.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Global Transposon Mutagenesis and Essential Gene Analysis of Helicobacter pylori{dagger}

Nina R. Salama,1* Benjamin Shepherd,1 and Stanley Falkow2

Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington,1 Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California2

Received 8 June 2004/ Accepted 23 August 2004

We have constructed a genome-saturating mutant library of the human gastric pathogen Helicobacter pylori. Microarray tracking of transposon mutants (MATT) allowed us to map the position of 5,363 transposon mutants in our library. While we generally found insertions well distributed throughout the genome, 344 genes had no detectable transposon insertions, and this list is predicted to be highly enriched for essential genes. Comparison to the essential gene set of other bacteria revealed a surprisingly limited overlap with all organisms tested (11%), while 55% were essential in some organisms but not others. We independently verified the essentiality of several gene products, including an HtrA family serine protease, a hypothetical protein with putative phospholipase D activity, and a riboflavin specific deaminase. A limited screen for motility mutants allowed us to estimate that 4.5% of the genome is dedicated to this virulence-associated phenotype.

* Corresponding author. Mailing address: Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Mailstop C3-168, P.O. Box 19024, Seattle, WA 98109-1024. Phone: (206) 667-1540. Fax: (206) 667-6524. E-mail: nsalama{at}fhcrc.org.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, December 2004, p. 7926-7935, Vol. 186, No. 23
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.23.7926-7935.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gawronski, J. D., Wong, S. M. S., Giannoukos, G., Ward, D. V., Akerley, B. J. (2009). Tracking insertion mutants within libraries by deep sequencing and a genome-wide screen for Haemophilus genes required in the lung. Proc. Natl. Acad. Sci. USA 106: 16422-16427 [Abstract] [Full Text]  
  • Zhang, R., Lin, Y. (2009). DEG 5.0, a database of essential genes in both prokaryotes and eukaryotes. Nucleic Acids Res 37: D455-D458 [Abstract] [Full Text]  
  • Castillo, A. R., Woodruff, A. J., Connolly, L. E., Sause, W. E., Ottemann, K. M. (2008). Recombination-Based In Vivo Expression Technology Identifies Helicobacter pylori Genes Important for Host Colonization. Infect. Immun. 76: 5632-5644 [Abstract] [Full Text]  
  • Boneca, I. G., Ecobichon, C., Chaput, C., Mathieu, A., Guadagnini, S., Prevost, M.-C., Colland, F., Labigne, A., de Reuse, H. (2008). Development of Inducible Systems To Engineer Conditional Mutants of Essential Genes of Helicobacter pylori. Appl. Environ. Microbiol. 74: 2095-2102 [Abstract] [Full Text]  
  • Reid, A. N., Pandey, R., Palyada, K., Whitworth, L., Doukhanine, E., Stintzi, A. (2008). Identification of Campylobacter jejuni Genes Contributing to Acid Adaptation by Transcriptional Profiling and Genome-Wide Mutagenesis. Appl. Environ. Microbiol. 74: 1598-1612 [Abstract] [Full Text]  
  • McLennan, M. K., Ringoir, D. D., Frirdich, E., Svensson, S. L., Wells, D. H., Jarrell, H., Szymanski, C. M., Gaynor, E. C. (2008). Campylobacter jejuni Biofilms Up-Regulated in the Absence of the Stringent Response Utilize a Calcofluor White-Reactive Polysaccharide. J. Bacteriol. 190: 1097-1107 [Abstract] [Full Text]  
  • Kong, Y., Wu, D., Bai, H., Han, C., Chen, J., Chen, L., Hu, L., Jiang, H., Shen, X. (2008). Enzymatic Characterization and Inhibitor Discovery of a New Cystathionine {gamma}-Synthase from Helicobacter pylori. J Biochem 143: 59-68 [Abstract] [Full Text]  
  • Carpenter, B. M., McDaniel, T. K., Whitmire, J. M., Gancz, H., Guidotti, S., Censini, S., Merrell, D. S. (2007). Expanding the Helicobacter pylori Genetic Toolbox: Modification of an Endogenous Plasmid for Use as a Transcriptional Reporter and Complementation Vector. Appl. Environ. Microbiol. 73: 7506-7514 [Abstract] [Full Text]  
  • Steyert, S. R., Pineiro, S. A. (2007). Development of a Novel Genetic System To Create Markerless Deletion Mutants of Bdellovibrio bacteriovorus. Appl. Environ. Microbiol. 73: 4717-4724 [Abstract] [Full Text]  
  • Weiss, D. S., Brotcke, A., Henry, T., Margolis, J. J., Chan, K., Monack, D. M. (2007). In vivo negative selection screen identifies genes required for Francisella virulence. Proc. Natl. Acad. Sci. USA 104: 6037-6042 [Abstract] [Full Text]  
  • Bijlsma, J. J. E., Burghout, P., Kloosterman, T. G., Bootsma, H. J., de Jong, A., Hermans, P. W. M., Kuipers, O. P. (2007). Development of Genomic Array Footprinting for Identification of Conditionally Essential Genes in Streptococcus pneumoniae. Appl. Environ. Microbiol. 73: 1514-1524 [Abstract] [Full Text]  
  • Ozaki, H., Ikeuchi, M., Ogawa, T., Fukuzawa, H., Sonoike, K. (2007). Large-Scale Analysis of Chlorophyll Fluorescence Kinetics in Synechocystis sp. PCC 6803: Identification of the Factors Involved in the Modulation of Photosystem Stoichiometry. Plant Cell Physiol 48: 451-458 [Abstract] [Full Text]  
  • Baldwin, D. N., Shepherd, B., Kraemer, P., Hall, M. K., Sycuro, L. K., Pinto-Santini, D. M., Salama, N. R. (2007). Identification of Helicobacter pylori Genes That Contribute to Stomach Colonization. Infect. Immun. 75: 1005-1016 [Abstract] [Full Text]  
  • Thomaides, H. B., Davison, E. J., Burston, L., Johnson, H., Brown, D. R., Hunt, A. C., Errington, J., Czaplewski, L. (2007). Essential Bacterial Functions Encoded by Gene Pairs. J. Bacteriol. 189: 591-602 [Abstract] [Full Text]  
  • Mouery, K., Rader, B. A., Gaynor, E. C., Guillemin, K. (2006). The Stringent Response Is Required for Helicobacter pylori Survival of Stationary Phase, Exposure to Acid, and Aerobic Shock.. J. Bacteriol. 188: 5494-5500 [Abstract] [Full Text]  
  • Bourzac, K. M., Satkamp, L. A., Guillemin, K. (2006). The Helicobacter pylori cag Pathogenicity Island Protein CagN Is a Bacterial Membrane-Associated Protein That Is Processed at Its C Terminus.. Infect. Immun. 74: 2537-2543 [Abstract] [Full Text]  
  • Liberati, N. T., Urbach, J. M., Miyata, S., Lee, D. G., Drenkard, E., Wu, G., Villanueva, J., Wei, T., Ausubel, F. M. (2006). An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc. Natl. Acad. Sci. USA 103: 2833-2838 [Abstract] [Full Text]  
  • Glass, J. I., Assad-Garcia, N., Alperovich, N., Yooseph, S., Lewis, M. R., Maruf, M., Hutchison, C. A. III, Smith, H. O., Venter, J. C. (2006). Essential genes of a minimal bacterium. Proc. Natl. Acad. Sci. USA 103: 425-430 [Abstract] [Full Text]  
  • Gancz, H., Censini, S., Merrell, D. S. (2006). Iron and pH Homeostasis Intersect at the Level of Fur Regulation in the Gastric Pathogen Helicobacter pylori. Infect. Immun. 74: 602-614 [Abstract] [Full Text]  
  • Fang, G., Rocha, E., Danchin, A. (2005). How Essential Are Nonessential Genes?. Mol Biol Evol 22: 2147-2156 [Abstract] [Full Text]  
  • Chan, K., Kim, C. C., Falkow, S. (2005). Microarray-Based Detection of Salmonella enterica Serovar Typhimurium Transposon Mutants That Cannot Survive in Macrophages and Mice. Infect. Immun. 73: 5438-5449 [Abstract] [Full Text]  
  • Lewenza, S., Falsafi, R. K., Winsor, G., Gooderham, W. J., McPhee, J. B., Brinkman, F. S.L., Hancock, R. E.W. (2005). Construction of a mini-Tn5-luxCDABE mutant library in Pseudomonas aeruginosa PAO1: A tool for identifying differentially regulated genes. Genome Res 15: 583-589 [Abstract] [Full Text]  
  • Moore, J. M., Salama, N. R. (2005). Mutational Analysis of Metronidazole Resistance in Helicobacter pylori. Antimicrob. Agents Chemother. 49: 1236-1237 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»