This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Haveman, S. A.
Right arrow Articles by Voordouw, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Haveman, S. A.
Right arrow Articles by Voordouw, G.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2004, p. 7944-7950, Vol. 186, No. 23
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.23.7944-7950.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Physiological and Gene Expression Analysis of Inhibition of Desulfovibrio vulgaris Hildenborough by Nitrite{dagger}

Shelley A. Haveman, E. Anne Greene, Claire P. Stilwell, Johanna K. Voordouw, and Gerrit Voordouw*

Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada

Received 11 June 2004/ Accepted 2 September 2004

A Desulfovibrio vulgaris Hildenborough mutant lacking the nrfA gene for the catalytic subunit of periplasmic cytochrome c nitrite reductase (NrfHA) was constructed. In mid-log phase, growth of the wild type in medium containing lactate and sulfate was inhibited by 10 mM nitrite, whereas 0.6 mM nitrite inhibited the nrfA mutant. Lower concentrations (0.04 mM) inhibited the growth of both mutant and wild-type cells on plates. Macroarray hybridization indicated that nitrite upregulates the nrfHA genes and downregulates genes for sulfate reduction enzymes catalyzing steps preceding the reduction of sulfite to sulfide by dissimilatory sulfite reductase (DsrAB), for two membrane-bound electron transport complexes (qmoABC and dsrMKJOP) and for ATP synthase (atp). DsrAB is known to bind and slowly reduce nitrite. The data support a model in which nitrite inhibits DsrAB (apparent dissociation constant Km for nitrite = 0.03 mM), and in which NrfHA (Km for nitrite = 1.4 mM) limits nitrite entry by reducing it to ammonia when nitrite concentrations are at millimolar levels. The gene expression data and consideration of relative gene locations suggest that QmoABC and DsrMKJOP donate electrons to adenosine phosphosulfate reductase and DsrAB, respectively. Downregulation of atp genes, as well as the recorded cell death following addition of inhibitory nitrite concentrations, suggests that the proton gradient collapses when electrons are diverted from cytoplasmic sulfate to periplasmic nitrite reduction.

* Corresponding author. Mailing address: Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada. Phone: (403) 220-6388. Fax: (403) 289-9311. E-mail: voordouw{at}ucalgary.ca.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, December 2004, p. 7944-7950, Vol. 186, No. 23
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.23.7944-7950.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Marietou, A., Griffiths, L., Cole, J. (2009). Preferential Reduction of the Thermodynamically Less Favorable Electron Acceptor, Sulfate, by a Nitrate-Reducing Strain of the Sulfate-Reducing Bacterium Desulfovibrio desulfuricans 27774. J. Bacteriol. 191: 882-889 [Abstract] [Full Text]  
  • Schwermer, C. U., Lavik, G., Abed, R. M. M., Dunsmore, B., Ferdelman, T. G., Stoodley, P., Gieseke, A., de Beer, D. (2008). Impact of Nitrate on the Structure and Function of Bacterial Biofilm Communities in Pipelines Used for Injection of Seawater into Oil Fields. Appl. Environ. Microbiol. 74: 2841-2851 [Abstract] [Full Text]  
  • Kosaka, T., Kato, S., Shimoyama, T., Ishii, S., Abe, T., Watanabe, K. (2008). The genome of Pelotomaculum thermopropionicum reveals niche-associated evolution in anaerobic microbiota. Genome Res 18: 442-448 [Abstract] [Full Text]  
  • Meyer, B., Kuever, J. (2007). Molecular analysis of the distribution and phylogeny of dissimilatory adenosine-5'-phosphosulfate reductase-encoding genes (aprBA) among sulfur-oxidizing prokaryotes. Microbiology 153: 3478-3498 [Abstract] [Full Text]  
  • Meyer, B., Kuever, J. (2007). Phylogeny of the alpha and beta subunits of the dissimilatory adenosine-5'-phosphosulfate (APS) reductase from sulfate-reducing prokaryotes - origin and evolution of the dissimilatory sulfate-reduction pathway. Microbiology 153: 2026-2044 [Abstract] [Full Text]  
  • Hubert, C., Voordouw, G. (2007). Oil Field Souring Control by Nitrate-Reducing Sulfurospirillum spp. That Outcompete Sulfate-Reducing Bacteria for Organic Electron Donors. Appl. Environ. Microbiol. 73: 2644-2652 [Abstract] [Full Text]  
  • Greene, E. A., Brunelle, V., Jenneman, G. E., Voordouw, G. (2006). Synergistic Inhibition of Microbial Sulfide Production by Combinations of the Metabolic Inhibitor Nitrite and Biocides. Appl. Environ. Microbiol. 72: 7897-7901 [Abstract] [Full Text]  
  • Haveman, S. A., Holmes, D. E., Ding, Y.-H. R., Ward, J. E., DiDonato, R. J. Jr., Lovley, D. R. (2006). c-Type Cytochromes in Pelobacter carbinolicus. Appl. Environ. Microbiol. 72: 6980-6985 [Abstract] [Full Text]  
  • Wildschut, J. D., Lang, R. M., Voordouw, J. K., Voordouw, G. (2006). Rubredoxin:Oxygen Oxidoreductase Enhances Survival of Desulfovibrio vulgaris Hildenborough under Microaerophilic Conditions.. J. Bacteriol. 188: 6253-6260 [Abstract] [Full Text]  
  • He, Q., Huang, K. H., He, Z., Alm, E. J., Fields, M. W., Hazen, T. C., Arkin, A. P., Wall, J. D., Zhou, J. (2006). Energetic Consequences of Nitrite Stress in Desulfovibrio vulgaris Hildenborough, Inferred from Global Transcriptional Analysis.. Appl. Environ. Microbiol. 72: 4370-4381 [Abstract] [Full Text]  
  • Redding, A. M., Mukhopadhyay, A., Joyner, D. C., Hazen, T. C., Keasling, J. D. (2006). Study of nitrate stress in Desulfovibrio vulgaris Hildenborough using iTRAQ proteomics. Brief Funct Genomic Proteomic 5: 133-143 [Abstract] [Full Text]  
  • Chhabra, S. R., He, Q., Huang, K. H., Gaucher, S. P., Alm, E. J., He, Z., Hadi, M. Z., Hazen, T. C., Wall, J. D., Zhou, J., Arkin, A. P., Singh, A. K. (2006). Global Analysis of Heat Shock Response in Desulfovibrio vulgaris Hildenborough.. J. Bacteriol. 188: 1817-1828 [Abstract] [Full Text]  
  • Mussmann, M., Richter, M., Lombardot, T., Meyerdierks, A., Kuever, J., Kube, M., Glockner, F. O., Amann, R. (2005). Clustered Genes Related to Sulfate Respiration in Uncultured Prokaryotes Support the Theory of Their Concomitant Horizontal Transfer. J. Bacteriol. 187: 7126-7137 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»