This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sherlock, O.
Right arrow Articles by Klemm, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sherlock, O.
Right arrow Articles by Klemm, P.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2004, p. 8058-8065, Vol. 186, No. 23
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.23.8058-8065.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Novel Roles for the AIDA Adhesin from Diarrheagenic Escherichia coli: Cell Aggregation and Biofilm Formation

Orla Sherlock,1 Mark A. Schembri,1,2 Andreas Reisner,1 and Per Klemm1*

Centre for Biomedical Microbiology, BioCentrum-DTU, Technical University of Denmark, Lyngby, Denmark,1 Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, Queensland, Australia2

Received 11 August 2004/ Accepted 13 August 2004

Diarrhea-causing Escherichia coli strains are responsible for numerous cases of gastrointestinal disease and constitute a serious health problem throughout the world. The ability to recognize and attach to host intestinal surfaces is an essential step in the pathogenesis of such strains. AIDA is a potent bacterial adhesin associated with some diarrheagenic E. coli strains. AIDA mediates bacterial attachment to a broad variety of human and other mammalian cells. It is a surface-displayed autotransporter protein and belongs to the selected group of bacterial glycoproteins; only the glycosylated form binds to mammalian cells. Here, we show that AIDA possesses self-association characteristics and can mediate autoaggregation of E. coli cells. We demonstrate that intercellular AIDA-AIDA interaction is responsible for bacterial autoaggregation. Interestingly, AIDA-expressing cells can interact with antigen 43 (Ag43)-expressing cells, which is indicative of an intercellular AIDA-Ag43 interaction. Additionally, AIDA expression dramatically enhances biofilm formation by E. coli on abiotic surfaces in flow chambers.

* Corresponding author. Mailing address: Centre for Biomedical Microbiology, BioCentrum-DTU, Bldg. 301, Technical University of Denmark, DK-2800 Lyngby, Denmark. Phone: 45 45 25 25 09. Fax: 45 45 93 28 09. E-mail: pkl{at}biocentrum.dtu.dk.

Journal of Bacteriology, December 2004, p. 8058-8065, Vol. 186, No. 23
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.23.8058-8065.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ong, C.-L. Y., Beatson, S. A., McEwan, A. G., Schembri, M. A. (2009). Conjugative Plasmid Transfer and Adhesion Dynamics in an Escherichia coli Biofilm. Appl. Environ. Microbiol. 75: 6783-6791 [Abstract] [Full Text]  
  • Jin, Y., Watt, R. M., Danchin, A., Huang, J.-d. (2009). Use of a Riboswitch-controlled Conditional Hypomorphic Mutation to Uncover a Role for the Essential csrA Gene in Bacterial Autoaggregation. J. Biol. Chem. 284: 28738-28745 [Abstract] [Full Text]  
  • Herold, S., Paton, J. C., Paton, A. W. (2009). Sab, a Novel Autotransporter of Locus of Enterocyte Effacement-Negative Shiga-Toxigenic Escherichia coli O113:H21, Contributes to Adherence and Biofilm Formation. Infect. Immun. 77: 3234-3243 [Abstract] [Full Text]  
  • Charbonneau, M.-E., Janvore, J., Mourez, M. (2009). Autoprocessing of the Escherichia coli AIDA-I Autotransporter: A NEW MECHANISM INVOLVING ACIDIC RESIDUES IN THE JUNCTION REGION. J. Biol. Chem. 284: 17340-17351 [Abstract] [Full Text]  
  • Karatan, E., Watnick, P. (2009). Signals, Regulatory Networks, and Materials That Build and Break Bacterial Biofilms. Microbiol. Mol. Biol. Rev. 73: 310-347 [Abstract] [Full Text]  
  • Vejborg, R. M., Klemm, P. (2009). Cellular chain formation in Escherichia coli biofilms. Microbiology 155: 1407-1417 [Abstract] [Full Text]  
  • Lawrenz, M. B., Lenz, J. D., Miller, V. L. (2009). A Novel Autotransporter Adhesin Is Required for Efficient Colonization during Bubonic Plague. Infect. Immun. 77: 317-326 [Abstract] [Full Text]  
  • Hart, E., Yang, J., Tauschek, M., Kelly, M., Wakefield, M. J., Frankel, G., Hartland, E. L., Robins-Browne, R. M. (2008). RegA, an AraC-Like Protein, Is a Global Transcriptional Regulator That Controls Virulence Gene Expression in Citrobacter rodentium. Infect. Immun. 76: 5247-5256 [Abstract] [Full Text]  
  • Valle, J., Mabbett, A. N., Ulett, G. C., Toledo-Arana, A., Wecker, K., Totsika, M., Schembri, M. A., Ghigo, J.-M., Beloin, C. (2008). UpaG, a New Member of the Trimeric Autotransporter Family of Adhesins in Uropathogenic Escherichia coli. J. Bacteriol. 190: 4147-4161 [Abstract] [Full Text]  
  • Charbonneau, M.-E., Mourez, M. (2007). Functional Organization of the Autotransporter Adhesin Involved in Diffuse Adherence. J. Bacteriol. 189: 9020-9029 [Abstract] [Full Text]  
  • Charbonneau, M.-E., Girard, V., Nikolakakis, A., Campos, M., Berthiaume, F., Dumas, F., Lepine, F., Mourez, M. (2007). O-Linked Glycosylation Ensures the Normal Conformation of the Autotransporter Adhesin Involved in Diffuse Adherence. J. Bacteriol. 189: 8880-8889 [Abstract] [Full Text]  
  • Greenberg, D. E., Porcella, S. F., Zelazny, A. M., Virtaneva, K., Sturdevant, D. E., Kupko, J. J. III, Barbian, K. D., Babar, A., Dorward, D. W., Holland, S. M. (2007). Genome Sequence Analysis of the Emerging Human Pathogenic Acetic Acid Bacterium Granulibacter bethesdensis. J. Bacteriol. 189: 8727-8736 [Abstract] [Full Text]  
  • Uzureau, S., Godefroid, M., Deschamps, C., Lemaire, J., De Bolle, X., Letesson, J.-J. (2007). Mutations of the Quorum Sensing-Dependent Regulator VjbR Lead to Drastic Surface Modifications in Brucella melitensis. J. Bacteriol. 189: 6035-6047 [Abstract] [Full Text]  
  • Ulett, G. C., Valle, J., Beloin, C., Sherlock, O., Ghigo, J.-M., Schembri, M. A. (2007). Functional Analysis of Antigen 43 in Uropathogenic Escherichia coli Reveals a Role in Long-Term Persistence in the Urinary Tract. Infect. Immun. 75: 3233-3244 [Abstract] [Full Text]  
  • Restieri, C., Garriss, G., Locas, M.-C., Dozois, C. M. (2007). Autotransporter-Encoding Sequences Are Phylogenetically Distributed among Escherichia coli Clinical Isolates and Reference Strains. Appl. Environ. Microbiol. 73: 1553-1562 [Abstract] [Full Text]  
  • Fexby, S., Bjarnsholt, T., Jensen, P. O., Roos, V., Hoiby, N., Givskov, M., Klemm, P. (2007). Biological Trojan Horse: Antigen 43 Provides Specific Bacterial Uptake and Survival in Human Neutrophils. Infect. Immun. 75: 30-34 [Abstract] [Full Text]  
  • Charbonneau, M.-E., Berthiaume, F., Mourez, M. (2006). Proteolytic Processing Is Not Essential for Multiple Functions of the Escherichia coli Autotransporter Adhesin Involved in Diffuse Adherence (AIDA-I). J. Bacteriol. 188: 8504-8512 [Abstract] [Full Text]  
  • Sebaihia, M., Preston, A., Maskell, D. J., Kuzmiak, H., Connell, T. D., King, N. D., Orndorff, P. E., Miyamoto, D. M., Thomson, N. R., Harris, D., Goble, A., Lord, A., Murphy, L., Quail, M. A., Rutter, S., Squares, R., Squares, S., Woodward, J., Parkhill, J., Temple, L. M. (2006). Comparison of the Genome Sequence of the Poultry Pathogen Bordetella avium with Those of B. bronchiseptica, B. pertussis, and B. parapertussis Reveals Extensive Diversity in Surface Structures Associated with Host Interaction.. J. Bacteriol. 188: 6002-6015 [Abstract] [Full Text]  
  • Ulett, G. C., Webb, R. I., Schembri, M. A. (2006). Antigen-43-mediated autoaggregation impairs motility in Escherichia coli. Microbiology 152: 2101-2110 [Abstract] [Full Text]  
  • Sherlock, O., Dobrindt, U., Jensen, J. B., Munk Vejborg, R., Klemm, P. (2006). Glycosylation of the Self-Recognizing Escherichia coli Ag43 Autotransporter Protein.. J. Bacteriol. 188: 1798-1807 [Abstract] [Full Text]  
  • Torres, A. G., Jeter, C., Langley, W., Matthysse, A. G. (2005). Differential Binding of Escherichia coli O157:H7 to Alfalfa, Human Epithelial Cells, and Plastic Is Mediated by a Variety of Surface Structures. Appl. Environ. Microbiol. 71: 8008-8015 [Abstract] [Full Text]  
  • Bullard, B., Lipski, S. L., Lafontaine, E. R. (2005). Hag Directly Mediates the Adherence of Moraxella catarrhalis to Human Middle Ear Cells. Infect. Immun. 73: 5127-5136 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»