Journal of Bacteriology, December 2004, p. 8058-8065, Vol. 186, No. 23
0021-9193/04/$08.00+0 DOI: 10.1128/JB.186.23.8058-8065.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
Novel Roles for the AIDA Adhesin from Diarrheagenic Escherichia coli: Cell Aggregation and Biofilm Formation
Orla Sherlock,1
Mark A. Schembri,1,2
Andreas Reisner,1 and
Per Klemm1*
Centre for Biomedical Microbiology, BioCentrum-DTU, Technical University of Denmark, Lyngby, Denmark,1
Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, Queensland, Australia2
Received 11 August 2004/
Accepted 13 August 2004
Diarrhea-causing Escherichia coli strains are responsible for numerous cases of gastrointestinal disease and constitute a serious health problem throughout the world. The ability to recognize and attach to host intestinal surfaces is an essential step in the pathogenesis of such strains. AIDA is a potent bacterial adhesin associated with some diarrheagenic E. coli strains. AIDA mediates bacterial attachment to a broad variety of human and other mammalian cells. It is a surface-displayed autotransporter protein and belongs to the selected group of bacterial glycoproteins; only the glycosylated form binds to mammalian cells. Here, we show that AIDA possesses self-association characteristics and can mediate autoaggregation of E. coli cells. We demonstrate that intercellular AIDA-AIDA interaction is responsible for bacterial autoaggregation. Interestingly, AIDA-expressing cells can interact with antigen 43 (Ag43)-expressing cells, which is indicative of an intercellular AIDA-Ag43 interaction. Additionally, AIDA expression dramatically enhances biofilm formation by E. coli on abiotic surfaces in flow chambers.
* Corresponding author. Mailing address: Centre for Biomedical Microbiology, BioCentrum-DTU, Bldg. 301, Technical University of Denmark, DK-2800 Lyngby, Denmark. Phone: 45 45 25 25 09. Fax: 45 45 93 28 09. E-mail: pkl{at}biocentrum.dtu.dk.
Journal of Bacteriology, December 2004, p. 8058-8065, Vol. 186, No. 23
0021-9193/04/$08.00+0 DOI: 10.1128/JB.186.23.8058-8065.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
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Copyright © 2004 by the American Society for Microbiology. All rights reserved.