This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mohd-Zain, Z.
Right arrow Articles by Crook, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mohd-Zain, Z.
Right arrow Articles by Crook, D. W.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2004, p. 8114-8122, Vol. 186, No. 23
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.23.8114-8122.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Transferable Antibiotic Resistance Elements in Haemophilus influenzae Share a Common Evolutionary Origin with a Diverse Family of Syntenic Genomic Islands

Zaini Mohd-Zain,1 Sarah L. Turner,2 Ana M. Cerdeño-Tárraga,3 Andrew K. Lilley,2 Thomas J. Inzana,4 A. Jane Duncan,4 Rosalind M. Harding,5 Derek W. Hood,6 Timothy E. Peto,1 and Derrick W. Crook1*

Infectious Diseases and Clinical Microbiology, Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital,1 Institute of Biological Anthropology,5 Molecular Infectious Diseases, Department of Paediatrics, Oxford University,6 Centre for Ecology and Hydrology, Oxford,2 The Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom,3 Virginia Polytechnic Institute and State University, Blacksburg, Virginia4

Received 7 May 2004/ Accepted 24 August 2004

Transferable antibiotic resistance in Haemophilus influenzae was first detected in the early 1970s. After this, resistance spread rapidly worldwide and was shown to be transferred by a large 40- to 60-kb conjugative element. Bioinformatics analysis of the complete sequence of a typical H. influenzae conjugative resistance element, ICEHin1056, revealed the shared evolutionary origin of this element. ICEHin1056 has homology to 20 contiguous sequences in the National Center for Biotechnology Information database. Systematic comparison of these homologous sequences resulted in identification of a conserved syntenic genomic island consisting of up to 33 core genes in 16 ß- and {gamma}-Proteobacteria. These diverse genomic islands shared a common evolutionary origin, insert into tRNA genes, and have diverged widely, with G+C contents ranging from 40 to 70% and amino acid homologies as low as 20 to 25% for shared core genes. These core genes are likely to account for the conjugative transfer of the genomic islands and may even encode autonomous replication. Accessory gene clusters were nestled among the core genes and encode the following diverse major attributes: antibiotic, metal, and antiseptic resistance; degradation of chemicals; type IV secretion systems; two-component signaling systems; Vi antigen capsule synthesis; toxin production; and a wide range of metabolic functions. These related genomic islands include the following well-characterized structures: SPI-7, found in Salmonella enterica serovar Typhi; PAP1 or pKLC102, found in Pseudomonas aeruginosa; and the clc element, found in Pseudomonas sp. strain B13. This is the first report of a diverse family of related syntenic genomic islands with a deep evolutionary origin, and our findings challenge the view that genomic islands consist only of independently evolving modules.

* Corresponding author. Mailing address: Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, University of Oxford, Headington, Oxford, OX3 9DU, United Kingdom. Phone: 44 1865 221226. Fax: 44 1865 764192. E-mail: derrick.crook{at}ndcls.ox.ac.uk.

Journal of Bacteriology, December 2004, p. 8114-8122, Vol. 186, No. 23
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.23.8114-8122.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Moxon, E. R. (2009). Bacterial variation, virulence and vaccines. Microbiology 155: 997-1003 [Abstract] [Full Text]  
  • Minoia, M., Gaillard, M., Reinhard, F., Stojanov, M., Sentchilo, V., van der Meer, J. R. (2008). Stochasticity and bistability in horizontal transfer control of a genomic island in Pseudomonas. Proc. Natl. Acad. Sci. USA 105: 20792-20797 [Abstract] [Full Text]  
  • Satola, S. W., Napier, B., Farley, M. M. (2008). Association of IS1016 with the hia Adhesin Gene and Biotypes V and I in Invasive Nontypeable Haemophilus influenzae. Infect. Immun. 76: 5221-5227 [Abstract] [Full Text]  
  • Klockgether, J., Wurdemann, D., Wiehlmann, L., Tummler, B. (2008). Transcript profiling of the Pseudomonas aeruginosa genomic islands PAGI-2 and pKLC102. Microbiology 154: 1599-1604 [Abstract] [Full Text]  
  • Baker, S., Pickard, D., Whitehead, S., Farrar, J., Dougan, G. (2008). Mobilization of the incQ Plasmid R300B with a Chromosomal Conjugation System in Salmonella enterica Serovar Typhi. J. Bacteriol. 190: 4084-4087 [Abstract] [Full Text]  
  • Gaillard, M., Pernet, N., Vogne, C., Hagenbuchle, O., van der Meer, J. R. (2008). Host and invader impact of transfer of the clc genomic island into Pseudomonas aeruginosa PAO1. Proc. Natl. Acad. Sci. USA 105: 7058-7063 [Abstract] [Full Text]  
  • Doungudomdacha, S., Volgina, A., DiRienzo, J. M. (2007). Evidence that the cytolethal distending toxin locus was once part of a genomic island in the periodontal pathogen Aggregatibacter (Actinobacillus) actinomycetemcomitans strain Y4. J Med Microbiol 56: 1519-1527 [Abstract] [Full Text]  
  • Chung, A., Perera, R., Brueggemann, A. B, Elamin, A. E, Harnden, A., Mayon-White, R., Smith, S., Crook, D. W, Mant, D. (2007). Effect of antibiotic prescribing on antibiotic resistance in individual children in primary care: prospective cohort study. BMJ 335: 429-429 [Abstract] [Full Text]  
  • Dimopoulou, I. D., Kartali, S. I., Harding, R. M., Peto, T. E. A., Crook, D. W. (2007). Diversity of antibiotic resistance integrative and conjugative elements among haemophili. J Med Microbiol 56: 838-846 [Abstract] [Full Text]  
  • Tristram, S., Jacobs, M. R., Appelbaum, P. C. (2007). Antimicrobial Resistance in Haemophilus influenzae. Clin. Microbiol. Rev. 20: 368-389 [Abstract] [Full Text]  
  • Klockgether, J., Wurdemann, D., Reva, O., Wiehlmann, L., Tummler, B. (2007). Diversity of the Abundant pKLC102/PAGI-2 Family of Genomic Islands in Pseudomonas aeruginosa. J. Bacteriol. 189: 2443-2459 [Abstract] [Full Text]  
  • Bellanger, X., Morel, C., Decaris, B., Guedon, G. (2007). Derepression of Excision of Integrative and Potentially Conjugative Elements from Streptococcus thermophilus by DNA Damage Response: Implication of a cI-Related Repressor. J. Bacteriol. 189: 1478-1481 [Abstract] [Full Text]  
  • Juhas, M., Crook, D. W., Dimopoulou, I. D., Lunter, G., Harding, R. M., Ferguson, D. J. P., Hood, D. W. (2007). Novel Type IV Secretion System Involved in Propagation of Genomic Islands. J. Bacteriol. 189: 761-771 [Abstract] [Full Text]  
  • Qiu, X., Gurkar, A. U., Lory, S. (2006). Interstrain transfer of the large pathogenicity island (PAPI-1) of Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA 103: 19830-19835 [Abstract] [Full Text]  
  • Kulasekara, B. R., Kulasekara, H. D., Wolfgang, M. C., Stevens, L., Frank, D. W., Lory, S. (2006). Acquisition and Evolution of the exoU Locus in Pseudomonas aeruginosa. J. Bacteriol. 188: 4037-4050 [Abstract] [Full Text]  
  • Gaillard, M., Vallaeys, T., Vorholter, F. J., Minoia, M., Werlen, C., Sentchilo, V., Puhler, A., van der Meer, J. R. (2006). The clc Element of Pseudomonas sp. Strain B13, a Genomic Island with Various Catabolic Properties.. J. Bacteriol. 188: 1999-2013 [Abstract] [Full Text]  
  • Erwin, A. L., Nelson, K. L., Mhlanga-Mutangadura, T., Bonthuis, P. J., Geelhood, J. L., Morlin, G., Unrath, W. C. T., Campos, J., Crook, D. W., Farley, M. M., Henderson, F. W., Jacobs, R. F., Muhlemann, K., Satola, S. W., van Alphen, L., Golomb, M., Smith, A. L. (2005). Characterization of Genetic and Phenotypic Diversity of Invasive Nontypeable Haemophilus influenzae. Infect. Immun. 73: 5853-5863 [Abstract] [Full Text]  
  • Harrison, A., Dyer, D. W., Gillaspy, A., Ray, W. C., Mungur, R., Carson, M. B., Zhong, H., Gipson, J., Gipson, M., Johnson, L. S., Lewis, L., Bakaletz, L. O., Munson, R. S. Jr. (2005). Genomic Sequence of an Otitis Media Isolate of Nontypeable Haemophilus influenzae: Comparative Study with H. influenzae Serotype d, Strain KW20. J. Bacteriol. 187: 4627-4636 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»