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Type II Isopentenyl Diphosphate Isomerase from Synechocystis sp. Strain PCC 6803

Department of Chemistry, University of Utah, Salt Lake City, Utah

Open reading frame sll1556 in the cyanobacterium Synechocystis sp. strain 6803 encodes a putative type II isopentenyl diphosphate (IPP) isomerase. The His₆-tagged protein was produced in Escherichia coli and purified by Ni²⁺ chromatography. The homotetrameric enzyme required NADPH, flavin mononucleotide, and Mg²⁺ for activity; K_m^IPP was 52 µM, and k_cat^IPP was 0.23 s^–1.